环状VAPA通过调控miR-615-3p和SMARCE1在视网膜母细胞瘤中的潜在肿瘤促进作用
The Potential Tumor Promotional Role of circVAPA in Retinoblastoma via Regulating miR-615-3p and SMARCE1.
作者信息
Xu Qibin
机构信息
Department of Ophthalmology, Zhejiang Hospital of Integrated Traditional Chinese and Western Medicine (Hangzhou Red Cross Hospital), Hangzhou, Zhejiang Province, People's Republic of China.
出版信息
Onco Targets Ther. 2020 Aug 5;13:7839-7849. doi: 10.2147/OTT.S254925. eCollection 2020.
BACKGROUND
Growing evidence reveals that circular RNAs (circRNAs) play roles in cancer development. However, the effects and possible mechanisms of circRNAs in retinoblastoma (RB) are far from clear.
METHODS
expression pattern was identified by RT-qPCR. induced effects on RB cells were tested by CCK-8, clone forming, flow cytometry and transwell assays. Bioinformatics assay, rescue experiments and dual-luciferase tests were applied for mechanism exploration. Additionally, mouse models were established for in vivo assays.
RESULTS
was upregulated in human RB specimen and RB cell lines, and was correlated with poor outcomes of Rb patients. Knockdown of could suppress the malignant phenotypes of RB. Mechanistic experiments demonstrated that could reverse the circVAPA induced effects on RB cells, and the downstream oncogene was positively regulated by circVAPA via . Further, in vivo analysis confirmed the findings.
CONCLUSION
In summary, promoted RB proliferation and metastasis by sponging , thereby upregulating . was a potential biomarker for Rb therapy.
背景
越来越多的证据表明，环状RNA（circRNAs）在癌症发展中发挥作用。然而，circRNAs在视网膜母细胞瘤（RB）中的作用及可能机制仍不清楚。
方法
通过RT-qPCR鉴定表达模式。通过CCK-8、克隆形成、流式细胞术和Transwell实验检测对RB细胞的诱导作用。应用生物信息学分析、挽救实验和双荧光素酶试验进行机制探索。此外，建立小鼠模型进行体内实验。
结果
在人RB标本和RB细胞系中上调，且与RB患者的不良预后相关。敲低可抑制RB的恶性表型。机制实验表明，可逆转circVAPA对RB细胞的诱导作用，下游癌基因通过circVAPA正向调控。此外，体内分析证实了这些发现。
结论
总之，通过海绵吸附促进RB增殖和转移，从而上调。是RB治疗的潜在生物标志物。
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