The First Clinical Medical College, Nanjing University of Chinese Medicine, Xianlin Road 138, Nanjing 210023, PR China.
Department of Head & Neck Surgery, The Affiliated Cancer Hospital of Nanjing Medical University & Jiangsu Cancer Hospital & Jiangsu Institute of Cancer Research, Baiziting 42, Nanjing 210029, PR China.
Epigenomics. 2020 Feb;12(4):303-317. doi: 10.2217/epi-2019-0124. Epub 2020 Jan 10.
We aimed to explore the roles of circular RNA, circVAPA in regulating cell migration and invasion of breast cancer. CircVAPA expression was detected in breast cancer tissues and cells. The role of circVAPA was evaluated by MTT assay, wound-healing and transwell assay. The relationship between circVAPA and miR-130a-5p and the location of circVAPA were explored. We discovered that circVAPA was dysregulated in breast cancer tissues and cells. Ectopic circVAPA regulated breast cancer migration, invasion and proliferation. CircVAPA was mainly expressed in the cytoplasm and could act as a miRNA sponge for miR-130a-5p, but did not regulate its parental gene. CircVAPA may promote migration and invasion capacity of breast cancer via harboring miR-130a-5p.
我们旨在探索环状 RNA，circVAPA 在调节乳腺癌细胞迁移和侵袭中的作用。检测乳腺癌组织和细胞中 circVAPA 的表达。通过 MTT 测定、划痕愈合和 Transwell 测定评估 circVAPA 的作用。探讨 circVAPA 与 miR-130a-5p 的关系及其定位。我们发现 circVAPA 在乳腺癌组织和细胞中失调。外源性 circVAPA 调节乳腺癌迁移、侵袭和增殖。circVAPA 主要表达在细胞质中，可作为 miR-130a-5p 的 miRNA 海绵，但不调节其亲本基因。circVAPA 可能通过携带 miR-130a-5p 促进乳腺癌的迁移和侵袭能力。
