Department of Immunology, Institute of Immunology and Transfusion Medicine, University Medicine Greifswald, Greifswald, Germany.
Department of Microbiology and Immunology, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt.
Front Immunol. 2020 Jul 31;11:1570. doi: 10.3389/fimmu.2020.01570. eCollection 2020.
In murine abdominal sepsis by colon ascendens stent peritonitis (CASP), a strong increase in serum IgM and IgG antibodies was observed, which reached maximum values 14 days following sepsis induction. The specificity of this antibody response was studied in serum and at the single cell level using a broad panel of bacterial, sepsis-unrelated as well as self-antigens. Whereas an antibacterial IgM/IgG response was rarely observed, studies at the single-cell level revealed that IgM antibodies, in particular, were largely polyreactive. Interestingly, at least 16% of the IgM mAbs and 20% of the IgG mAbs derived from post-septic mice showed specificity for oxidation-specific epitopes (OSEs), which are known targets of the innate/adaptive immune response. This identifies those self-antigens as the main target of B cell responses in sepsis.
在经升结肠支架腹膜炎（colon ascendens stent peritonitis，CASP）诱导的小鼠腹腔脓毒症模型中，观察到血清 IgM 和 IgG 抗体强烈增加，在脓毒症诱导后 14 天达到最大值。使用广泛的细菌、与脓毒症无关的以及自身抗原的面板，在血清和单细胞水平研究了这种抗体反应的特异性。虽然很少观察到针对细菌的 IgM/IgG 反应，但单细胞水平的研究表明，IgM 抗体特别是多反应性的。有趣的是，至少 16%的来源于脓毒症后小鼠的 IgM mAbs 和 20%的 IgG mAbs 显示出对氧化特异性表位（oxidation-specific epitopes，OSEs）的特异性，OSEs 是先天/适应性免疫反应的已知靶标。这表明这些自身抗原是脓毒症中 B 细胞反应的主要靶标。
