Sherwood J A, Spitalnik S L, Suarez S C, Marsh K, Howard R J
Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.
J Protozool. 1988 Feb;35(1):169-72. doi: 10.1111/j.1550-7408.1988.tb04098.x.
Plasmodium falciparum-infected erythrocytes were metabolically labeled with tritiated glucosamine. Lipid extracts were analyzed by high-performance thin-layer chromatography to compare labeled molecules of eleven isolates from patients, six cytoadherent in vitro strains, and two knobbed and two knobless strains from Aotus monkeys. Up to nineteen labeled bands were identified. Glycolipid GL1, previously identified in Malayan Camp, was present in all isolates and strains. Other molecules, between CG and GM1 and between GM1 and GD1a, varied in mobility or presence. There was no apparent association between labeled molecules and the presence of knobs or the property of cytoadherence.
用氚标记的葡糖胺对感染恶性疟原虫的红细胞进行代谢标记。通过高效薄层色谱分析脂质提取物，以比较来自患者的11株分离株、6株体外细胞黏附株以及来自夜猴的2株有突环和2株无突环菌株的标记分子。共鉴定出多达19条标记带。先前在马来亚营地鉴定出的糖脂GL1存在于所有分离株和菌株中。其他位于CG和GM1之间以及GM1和GD1a之间的分子，在迁移率或存在情况上有所不同。标记分子与突环的存在或细胞黏附特性之间没有明显关联。
