National Citrus Engineering and Technology Research Center, Citrus Research Institute, Southwest University, Chongqing, China.
State Cultivation Base of Crop Stress Biology for Southern Mountainous Land, Academy of Agricultural Sciences, Southwest University, Chongqing, China.
Arch Virol. 2020 Nov;165(11):2703-2707. doi: 10.1007/s00705-020-04786-1. Epub 2020 Aug 26.
A novel cytorhabdovirus, tentatively named "paper mulberry mosaic-associated virus" (PMuMaV), was discovered and identified by transcriptome sequencing (RNA-seq), small RNA sequencing (sRNA-seq), and RT-PCR amplification. The whole-genome sequence of PMuMaV is 13,736 nucleotides (nt) in length and contains six open reading frames (ORFs) encoding a nucleocapsid protein (N), a phosphoprotein (P), a putative movement protein (P3), a matrix protein (M), a glycoprotein (G), and an RNA-dependent RNA polymerase (L). The coding sequences are flanked by a 194-nt leader and a 370-nt trailer sequence at the 3' terminus and 5' terminus, respectively. Pairwise sequence comparisons showed that PMuMaV is related to northern cereal mosaic virus (NCMV, 38.97%), barley yellow striate mosaic virus (BYSMV, 38.86%), and maize yellow striate virus (MYSV, 38.76%), and phylogenetic analysis also placed these viruses together into the same branch, thus suggesting that PMuMaV is a member of a new species in the genus Cytorhabdovirus.
一种新的细胞弹状病毒，暂命名为“桑树花叶-associated 病毒”（PMuMaV），通过转录组测序（RNA-seq）、小 RNA 测序（sRNA-seq）和 RT-PCR 扩增被发现和鉴定。PMuMaV 的全基因组序列长 13736 个核苷酸（nt），包含六个开放阅读框（ORFs），编码核衣壳蛋白（N）、磷蛋白（P）、假定运动蛋白（P3）、基质蛋白（M）、糖蛋白（G）和 RNA 依赖性 RNA 聚合酶（L）。编码序列分别被 194nt 的 5'端前导序列和 370nt 的 3'端尾随序列所包围。序列比对表明，PMuMaV 与北方谷物花叶病毒（NCMV，38.97%）、大麦黄花条纹花叶病毒（BYSMV，38.86%）和玉米黄花条纹病毒（MYSV，38.76%）有亲缘关系，系统进化分析也将这些病毒归为同一分支，表明 PMuMaV 是细胞弹状病毒属的一个新种成员。
