Mahnashi Mater H
Department of Pharmaceutical Chemistry, College of Pharmacy, Najran University, Najran, Saudi Arabia.
Mikrochim Acta. 2020 Aug 27;187(9):519. doi: 10.1007/s00604-020-04523-0.
An innovative electrochemical nanoprobe was developed for determination of vortioxetine (VORT), a serotonergic antidepressant drug, for the first time. The fabrication of the nanoprobe is based on decoration of a glassy carbon electrode with three-dimensional nickel ferrite nanospheres modified activated graphite nanoplatelets (3D NiFeO NS/AGNP/GCE). The morphological characterization of the nanoprobe was carried out via scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FT-IR), Raman spectroscopy, energy dispersive X-ray spectroscopy (EDS), N-adsorption-desorption isotherm, and powder X-ray spectroscopy (PXRD). In addition, the electrochemical behavior of the nanoprobe was described using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS). A well-defined and irreversible peak at 0.82 V was seen at the surface of 3D NiFeO NS/AGNP/GCE. The proposed nanoprobe exhibited outstanding electro-catalytic activity towards VORT oxidation. Under the optimized conditions, the anodic oxidation currents were linearly proportional to VORT concentration at the working range 1.8-90 nM with a LOD of 0.55 nM. The nanoprobe was used to determine VORT in pharmaceutical tablets and human plasma samples. Satisfactory recoveries and RSD percentages were obtained in the range 103.8-107.7% (RSD% = 2.7-3.1%) and 101.4-105.3% (RSD % = 2.8-3.4%) for tablets and plasma samples, respectively. Moreover, the method was used to monitor VORT during a pharmacokinetic study in human volunteers with satisfactory results. The 3D NiFeO NS/AGNP/GCE shows excellent sensitivity, reproducibility, and selectivity towards VORT detection. The proposed electrode could be utilized as simple, rapid, and inexpensive sensing tool for routine analysis and during pharmacokinetic/pharmacodynamic investigations. Graphical abstract.
首次开发了一种创新的电化学纳米探针用于测定血清素能抗抑郁药物伏硫西汀（VORT）。该纳米探针的制备基于用三维镍铁氧体纳米球修饰的活性石墨纳米片（3D NiFeO NS/AGNP）对玻碳电极进行修饰。通过扫描电子显微镜（SEM）、透射电子显微镜（TEM）、傅里叶变换红外光谱（FT-IR）、拉曼光谱、能量色散X射线光谱（EDS）、N吸附-脱附等温线和粉末X射线光谱（PXRD）对纳米探针进行了形态表征。此外，使用循环伏安法（CV）、差分脉冲伏安法（DPV）和电化学阻抗谱（EIS）描述了纳米探针的电化学行为。在3D NiFeO NS/AGNP/GCE表面观察到在0.82 V处有一个明确的不可逆峰。所提出的纳米探针对VORT氧化表现出出色的电催化活性。在优化条件下，阳极氧化电流在1.8 - 90 nM的工作范围内与VORT浓度呈线性比例关系，检测限为0.55 nM。该纳米探针用于测定药片中的VORT和人体血浆样品。片剂和血浆样品的回收率和相对标准偏差（RSD）百分比分别在103.8 - 107.7%（RSD% = 2.7 - 3.1%）和101.4 - 105.3%（RSD% = 2.8 - 3.4%）范围内，结果令人满意。此外，该方法用于监测人体志愿者药代动力学研究期间的VORT，结果令人满意。3D NiFeO NS/AGNP/GCE对VORT检测显示出优异的灵敏度、重现性和选择性。所提出的电极可作为简单、快速且廉价的传感工具用于常规分析以及药代动力学/药效学研究。图形摘要。
