Department of Urology, Urological Research Laboratory, Translational UroOncology, University Hospital Düsseldorf, Düsseldorf, Germany.
Methods Mol Biol. 2021;2195:77-83. doi: 10.1007/978-1-0716-0860-9_6.
The hanging drop cell culture technique allows to study three-dimensional growth and differentiation of cell aggregates, that is, embryonic stem cells. Compared to standard two-dimensional monolayer cell cultivation, hanging drops allow for a better visualization and understanding of the developmental processes in vitro. Hanging drop cultivation can also be used to study biology of cancer cells three-dimensionally in vitro. This method can serve as an intermediate between the two-dimensional monolayer cell culture and in vivo models, which can be simply established in laboratories exhibiting minimum requirements of cell culture equipment. In this chapter, we describe the three-dimensional cultivation of germ cell cancer cell lines in hanging drops.
悬滴细胞培养技术允许研究细胞聚集体（即胚胎干细胞）的三维生长和分化。与标准的二维单层细胞培养相比，悬滴培养法可以更好地观察和理解体外发育过程。悬滴培养也可用于在体外研究癌细胞的三维生物学。这种方法可以作为二维单层细胞培养和体内模型之间的中间环节，这些模型可以在仅具备最低细胞培养设备要求的实验室中简单建立。在本章中，我们描述了在悬滴中培养生殖细胞癌细胞系的三维培养。
