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[从智利比奥比奥地区海岸采集的阿托尔贻贝样本中检测微小隐孢子虫(顶复门:隐孢子虫科)卵囊]

[Detection of Cryptosporidium parvum (Apicomplexan: Cryptosporidiidae) oocyst in Aulacomya ater specimen, extracted from to the coast Bio Bio Region, Chile].

作者信息

Suárez R Pilar, Yáñez D María José, Fernández F Ítalo, Madrid V Verónica

机构信息

Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción, Chile.

Departamento de Zoología, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile.

出版信息

Rev Chilena Infectol. 2020 Jun;37(3):257-262. doi: 10.4067/s0716-10182020000300257.

DOI:10.4067/s0716-10182020000300257
PMID:32853317
Abstract

BACKGROUND

The bivalve Aulacomya ater (cholga), is one of the most consumed mollusks by the population. However, there is evidence of fecal water contamination caused by causes that affect the sea, increasing the probability of contamination by the Cryptosporidium parvum, which generates cryptosporidiosis in people.

AIM

To determine the presence of C. parvum in cholga extracted from the Bio Bio Region (Chile).

METHODS

Fifty-five cholgas were selected from a cultivation center and a natural extraction bank. These samples were processed in the laboratory and the presence of acid-alcohol resistant elements was evaluated. Positive samples were analyzed by direct immunofluorescence with anti-C. parvum antibody.

RESULTS

16.4% of the total samples were affected by the oocysts of C.parvum.

CONCLUSIONS

For the first time we described C. parvum in A. ater from the Chilean coast, being this mollusk a possible vehicle for transmission of cryptosporidiosis to the population and their predatory animals. Furthermore, the presence of C. parvum reflects fecal water contamination on the evaluated coasts. We are currently monitoring other extraction areas for this mollusk.

摘要

背景

双壳贝类智利偏顶蛤是人们食用最多的软体动物之一。然而,有证据表明,受影响海洋的因素导致粪便水污染,增加了微小隐孢子虫污染的可能性,微小隐孢子虫会在人群中引发隐孢子虫病。

目的

确定从智利比奥比奥地区提取的智利偏顶蛤中微小隐孢子虫的存在情况。

方法

从一个养殖中心和一个天然捕捞库中选取了55只智利偏顶蛤。这些样本在实验室进行处理,并评估耐酸酒精元素的存在情况。对阳性样本用抗微小隐孢子虫抗体进行直接免疫荧光分析。

结果

总样本的16.4%受到微小隐孢子虫卵囊的影响。

结论

我们首次在智利海岸的智利偏顶蛤中描述了微小隐孢子虫,这种软体动物可能是隐孢子虫病传播给人群及其捕食动物的载体。此外,微小隐孢子虫的存在反映了所评估海岸的粪便水污染情况。我们目前正在监测这种软体动物的其他捕捞区域。

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