Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, 38000, Pakistan.
Department of Medicine, Cholistan University of Veterinary and Animal Sciences, Bahawalpur, 63100, Pakistan.
Microb Pathog. 2020 Nov;148:104450. doi: 10.1016/j.micpath.2020.104450. Epub 2020 Aug 25.
We evaluated three types of total six preparations against multidrug resistant E. coli i) three antibiotic coated ZnO nanoparticles (gentamicin coated nanoparticle-GNp; chloramphenicol coated nanoparticles-CNp; and both gentamicin & chloramphenicol coated nanoparticle-GCNp), ii) ZnO nanoparticle alone-Np, and iii) two antibiotics used in single (Gentamicin-G; and Chloramphenicol-C). A total of n = 200 sub-clinically positive mastitic milk samples of bovine origin were processed for isolation of MDR E. coli using microbiological and clinical laboratory & standard institute's protocols. ZnO Nps were prepared from zinc acetate dihydrate (Zn (CHCOO). 2HO), polyethylene glycol (CHO), and urea (CH₄N₂O) by standard chemical protocol. Nps were characterized by XRD and STEM analyses while coating of antibiotics on Nps was confirmed by UV-Visible spectrophotometric analysis. Analysis of variance and student t-test were applied at 5% probability using SPSS version 22 statistical software for inferences on obtained data. There was significantly (p < 0.05) lowest minimum inhibitory concentrations (MICs) and highest zone of inhibitions (ZOIs) in case of GCNp (10.42 ± 4.51 μg/mL & 22.00 ± 1.00 mm) followed by GNp (20.79 ± 8.95 μg/mL & 20.00 ± 1.00 mm) and then CNp (25.96 ± 8.95 μg/mL & 12.33 ± 0.57 mm). Percentage increase in ZOI were expressed as 135.8, 78.43, and 312.76% by GCNp when compared with that of G, C, and Np, respectively. GNp and CNp coated preparations exhibited 114.36 and 275.73% increase in ZOI than to that of G and C, respectively. Similar trend was found in percentage reduction of MICs of preparations. Highest filamentation, indicator of bacterial damage, of E. coli was noted at MIC of GCNp followed by GNp and CNp. The study concluded antibiotic coated ZnO nanoparticles significant candidates modulating antibiotic resistance in MDR E. coli.
i）三种抗生素涂层氧化锌纳米粒子（庆大霉素涂层纳米粒子-GNp；氯霉素涂层纳米粒子-CNp；以及庆大霉素和氯霉素双重涂层纳米粒子-GCNp）；ii）单独的氧化锌纳米粒子-Np；iii）两种抗生素单独使用（庆大霉素-G；和氯霉素-C）。总共处理了 n=200 份来自牛的亚临床阳性乳腺炎牛奶样本，使用微生物学和临床实验室及标准研究所的方案分离多药耐药大肠杆菌。氧化锌纳米粒子由三水合乙酸锌（Zn（CHCOO）.2HO）、聚乙二醇（CHO）和尿素（CH₄N₂O）通过标准化学方法制备。通过 XRD 和 STEM 分析对纳米粒子进行了表征，通过紫外可见分光光度分析证实了抗生素在纳米粒子上的涂层。使用 SPSS 版本 22 统计软件对获得的数据进行方差分析和学生 t 检验，置信度为 5%。在 GCNp（10.42±4.51μg/mL 和 22.00±1.00mm）的情况下，最低最小抑菌浓度（MICs）和最高抑菌区（ZOIs）显著（p<0.05），其次是 GNp（20.79±8.95μg/mL 和 20.00±1.00mm），然后是 CNp（25.96±8.95μg/mL 和 12.33±0.57mm）。与 G、C 和 Np 相比，GCNp 的抑菌区（ZOI）增加了 135.8%、78.43%和 312.76%。GNp 和 CNp 涂层制剂的抑菌区（ZOI）比 G 和 C 分别增加了 114.36%和 275.73%。MIC 时的抑菌区（ZOI）也有类似的增加趋势。制剂 MICs 的降低百分比。GCNp 时大肠杆菌的丝状化程度最高，表明细菌损伤最大，其次是 GNp 和 CNp。研究得出结论，抗生素涂层氧化锌纳米粒子是调节多药耐药大肠杆菌抗生素耐药性的有前途的候选物。
