Biofuels Institute, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, China; Botany Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Department of Microbiology, Faculty of Pharmacy Tanta University, Tanta, 31527, Egypt.
Microb Pathog. 2020 Jun;143:104164. doi: 10.1016/j.micpath.2020.104164. Epub 2020 Mar 19.
Escherichia coli is a major global foodborne pathogen, infecting a wide range of animals and contaminating their meat products. E. coli, can lead to high morbidity and mortality with a huge economic loss especially if foodborne diseases are associated with multidrug resistant (MDR)- and multivirulent-producing pathogens. Due to the increased resistance to common antimicrobials used to treat livestock animals and human infections, the discovery of new and innovative nanomaterials are in high demand. Recently, metal oxides can be considered as effective inorganic agents with antimicrobial features. Hence, this study might be the first to evaluate the efficiency of metal oxide nanoparticles (MO-NPs) as novel antibacterial agents against MDR/multivirulent E. coli pathogens isolated from chicken meat. The occurrence of pathogenic E. coli was determined in fresh warm chicken meat parts (breast, thigh, liver and gizzard). Ninety-one of 132 (69%) chicken meat parts were Escherichia -positive with E. coli as the only species isolated. Out of identified 240 E. coli strains, 72.5% (174/240) were classified as MDR E. coli strains. Fifty-five profile patterns were obtained. From each pattern, one strain was randomly selected for further analysis of virulence and resistance genes. Extracted DNA was assessed for the presence of antibiotic resistance genes (bla, bla, bla, bla, bla, tetA, aadA, and aac(3)-IV) and virulence genes (stx1, stx2, hlyA, eaeA, aggR, eltB, estIb, papA, afa and hlyD). Clustering analyses revealed that 10 E. coli harboring the highest number of virulence and resistance genes were shifted together into one cluster designated as cluster X. The average activities of zinc peroxide nanoparticles (ZnO-NPs) were higher than that of zinc oxide nanoparticles (ZnO-NPs) and titanium dioxide nanoparticles (TiO-NPs) by 20% and 29%, respectively. The anti-inflammatory activity of ZnO-NPs in comparison with aspirin was assessed using membrane stabilization, albumin denaturation, and proteinase inhibition methods. Significant anti-inflammatory activity of ZnO-NPs was achieved at concentration levels of 500-1000 μg/ml. It seems that MO-NPs are effective alternative agents, since they exhibited a competitive antibacterial capability against MDR/multivirulent-producing E. coli pathogens isolated from chicken meat. Hence, ZnO-NPs are a promising nanoparticles-based material for controlling foodborne pathogens, thereby valued for food safety applications.
大肠杆菌是一种主要的全球食源性致病菌，感染范围广泛的动物并污染其肉类产品。大肠杆菌可导致高发病率和死亡率，造成巨大的经济损失，尤其是如果食源性疾病与多药耐药（MDR）和多毒力产生的病原体有关。由于用于治疗牲畜和人类感染的常用抗菌药物的耐药性增加，对新型创新纳米材料的需求很高。最近，金属氧化物可用作具有抗菌特性的有效无机试剂。因此，本研究可能是首次评估金属氧化物纳米颗粒（MO-NPs）作为新型抗药性/多毒力大肠杆菌病原体的新型抗菌剂对鸡肉分离物的效率。在新鲜温热的鸡肉部分（乳房、大腿、肝脏和肫）中确定了致病性大肠杆菌的发生。132 个鸡肉部分中有 91 个（69％）呈大肠杆菌阳性，仅分离出大肠杆菌。在鉴定的 240 株大肠杆菌中，72.5％（174/240）被分类为多药耐药大肠杆菌菌株。得到了 55 种图谱模式。从每种模式中，随机选择一个菌株进行进一步的毒力和耐药基因分析。提取的 DNA 用于评估抗生素耐药基因（bla、bla、bla、bla、bla、tetA、aadA 和 aac(3)-IV）和毒力基因（stx1、stx2、hlyA、eaeA、aggR、eltB、estIb、papA、afa 和 hlyD）的存在。聚类分析表明，携带最高数量毒力和耐药基因的 10 株大肠杆菌被一起转移到一个命名为簇 X 的簇中。氧化锌纳米颗粒（ZnO-NPs）的平均活性比氧化锌纳米颗粒（ZnO-NPs）和二氧化钛纳米颗粒（TiO-NPs）分别高 20％和 29％。与阿司匹林相比，用膜稳定、白蛋白变性和蛋白酶抑制方法评估了 ZnO-NPs 的抗炎活性。在 500-1000μg/ml 的浓度水平下，ZnO-NPs 表现出显著的抗炎活性。似乎 MO-NPs 是有效的替代剂，因为它们对从鸡肉中分离出的多药耐药/多毒力产生的大肠杆菌病原体表现出竞争性的抗菌能力。因此，ZnO-NPs 是一种有前途的基于纳米材料的材料，可用于控制食源性病原体，从而在食品安全应用中具有价值。
