Wen Kai, Bai Yuchen, Wei Yujie, Li Chenglong, Shen Jianzhong, Wang Zhanhui
Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Veterinary Medicine, China Agricultural University, Beijing Key Laboratory of Detection Technology for Animal Derived Food Safety Beijing Laboratory for Food Quality and Safety, 100193 Beijing, People's Republic of China.
J Agric Food Chem. 2020 Sep 30;68(39):10944-10950. doi: 10.1021/acs.jafc.0c04333. Epub 2020 Sep 3.
Antibodies with high titer and affinity to small molecules are critical in the field of vaccines against drugs of abuse, antidotes to toxins, and immunoassays for compounds. However, little is known regarding how properties of small molecules have influence and which molecular descriptors could indicate the degree of the antibody response. On the basis of our previous study, we designed and synthesized two groups of hapten molecules with varied hydrophobicity to investigate the relationship between the properties of the small molecules and the antibody response in terms of titer and affinity. We found that the magnitude of the antibody response was positively correlated with the degree of molecular hydrophobicity and related descriptors. This study provides insight into the immunological characteristics of small molecules themselves and useful clues to produce high-quality antibodies against small molecules.
对小分子具有高滴度和亲和力的抗体在抗滥用药物疫苗、毒素解毒剂以及化合物免疫分析等领域至关重要。然而，关于小分子的性质如何产生影响以及哪些分子描述符可以表明抗体反应的程度，我们知之甚少。基于我们之前的研究，我们设计并合成了两组具有不同疏水性的半抗原分子，以研究小分子性质与抗体反应在滴度和亲和力方面的关系。我们发现抗体反应的强度与分子疏水性程度和相关描述符呈正相关。这项研究深入了解了小分子自身的免疫特性，并为生产针对小分子的高质量抗体提供了有用的线索。
