Solaris National Synchrotron Radiation Centre, Jagiellonian University, Czerwone Maki 98, Krakow 30-392, Poland.
Institute of Nuclear Physics, Polish Academy of Sciences, Krakow PL-31342, Poland.
Anal Chem. 2020 Oct 6;92(19):13313-13318. doi: 10.1021/acs.analchem.0c02591. Epub 2020 Sep 11.
Fourier transform infrared spectroscopy has emerged as a powerful tool for tissue specimen investigation. Its nondestructive and label-free character enables direct determination of biochemical composition of samples. Furthermore, the introduction of polarization enriches this technique by the possibility of molecular orientation study apart from purely quantitative analysis. Most of the molecular orientation studies focused on polymer samples with a well-defined molecular axis. Here, a four-polarization approach for Herman's in-plane orientation function and azimuthal angle determination was applied to a human tissue sample investigation for the first time. Attention was focused on fibrous tissues rich in collagen because of their cylindrical shape and established amide bond vibrations. Despite the fact that the tissue specimen contains a variety of molecules, the presented results of molecular ordering and orientation agree with the theoretical prediction based on sample composition and vibration directions.
傅里叶变换红外光谱学已成为组织标本研究的有力工具。其无损和无标记的特点使其能够直接测定样品的生物化学成分。此外，偏振的引入通过除了纯粹的定量分析之外还可以研究分子取向，从而丰富了这项技术。大多数分子取向研究都集中在具有明确分子轴的聚合物样品上。在这里，首次将赫尔曼面内取向函数和方位角的四偏振方法应用于人体组织样品的研究。由于富含胶原蛋白的纤维组织具有圆柱形形状和已建立的酰胺键振动，因此引起了人们的关注。尽管组织标本包含多种分子，但所呈现的分子有序性和取向的结果与基于样品组成和振动方向的理论预测相符。
