拉曼和傅里叶变换红外（FT-IR）矿物与基质比与模型化合物和天然骨组织的物理化学性质相关。
Raman and Fourier Transform Infrared (FT-IR) Mineral to Matrix Ratios Correlate with Physical Chemical Properties of Model Compounds and Native Bone Tissue.
机构信息
1 Sibley School of Mechanical and Aerospace Engineering, Cornell University, Ithaca, NY, USA.
2 Department of Materials Science and Engineering, Cornell University, Ithaca, NY, USA.
出版信息
Appl Spectrosc. 2017 Oct;71(10):2404-2410. doi: 10.1177/0003702817709286. Epub 2017 Aug 1.
Raman and Fourier transform infrared (FT-IR) spectroscopic imaging techniques can be used to characterize bone composition. In this study, our objective was to validate the Raman mineral:matrix ratios (ν PO:amide III, ν PO:amide I, ν PO:Proline + hydroxyproline, ν PO:Phenylalanine, ν PO:δ CH peak area ratios) by correlating them to ash fraction and the IR mineral:matrix ratio (ν PO:amide I peak area ratio) in chemical standards and native bone tissue. Chemical standards consisting of varying ratios of synthetic hydroxyapatite (HA) and collagen, as well as bone tissue from humans, sheep, and mice, were characterized with confocal Raman spectroscopy and FT-IR spectroscopy and gravimetric analysis. Raman and IR mineral:matrix ratio values from chemical standards increased reciprocally with ash fraction (Raman ν PO/Amide III: P < 0.01, R= 0.966; Raman ν PO/Amide I: P < 0.01, R= 0.919; Raman ν PO/Proline + Hydroxyproline: P < 0.01, R= 0.976; Raman ν PO/Phenylalanine: P < 0.01, R= 0.911; Raman ν PO/δ CH: P < 0.01, R= 0.894; IR P < 0.01, R= 0.91). Fourier transform infrared mineral:matrix ratio values from native bone tissue were also similar to theoretical mineral:matrix ratio values for a given ash fraction. Raman and IR mineral:matrix ratio values were strongly correlated ( P < 0.01, R= 0.82). These results were confirmed by calculating the mineral:matrix ratio for theoretical IR spectra, developed by applying the Beer-Lambert law to calculate the relative extinction coefficients of HA and collagen over the same range of wavenumbers (800-1800 cm). The results confirm that the Raman mineral:matrix bone composition parameter correlates strongly to ash fraction and to its IR counterpart. Finally, the mineral:matrix ratio values of the native bone tissue are similar to those of both chemical standards and theoretical values, confirming the biological relevance of the chemical standards and the characterization techniques.
拉曼和傅里叶变换红外（FT-IR）光谱成象技术可用于描述骨成分。在本研究中，我们的目的是通过将拉曼矿物与基质比值（νPO：酰胺 III、νPO：酰胺 I、νPO：脯氨酸+羟脯氨酸、νPO：苯丙氨酸、νPO：δ CH 峰面积比值）与灰分分数以及化学标准品和天然骨组织中的 IR 矿物与基质比值（νPO：酰胺 I 峰面积比值）相关联来验证这些比值。由不同比例的合成羟磷灰石（HA）和胶原蛋白组成的化学标准品以及来自人类、绵羊和小鼠的骨组织，通过共焦拉曼光谱和 FT-IR 光谱以及重量分析进行了特征描述。化学标准品的拉曼和 IR 矿物与基质比值随灰分分数呈反向变化（Raman νPO/酰胺 III：P<0.01，R=0.966；Raman νPO/酰胺 I：P<0.01，R=0.919；Raman νPO/脯氨酸+羟脯氨酸：P<0.01，R=0.976；Raman νPO/苯丙氨酸：P<0.01，R=0.911；Raman νPO/δ CH：P<0.01，R=0.894；IR P<0.01，R=0.91）。天然骨组织的 FT-IR 矿物与基质比值也与给定灰分分数的理论矿物与基质比值相似。拉曼和 IR 矿物与基质比值具有很强的相关性（P<0.01，R=0.82）。通过应用 Beer-Lambert 定律计算 HA 和胶原蛋白在相同波数范围内（800-1800 cm）的相对消光系数，为理论 IR 光谱计算矿物与基质比值，从而证实了这一结果。这些结果证实，拉曼矿物与基质骨成分参数与灰分分数及其 IR 对应物密切相关。最后，天然骨组织的矿物与基质比值与化学标准品和理论值相似，这证实了化学标准品和特征描述技术的生物学相关性。
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