Laboratoire de Chimie et Biologie des Membranes et Nano-objets, Université de Bordeaux, CNRS UMR 5084, Bordeaux, France.
Anal Bioanal Chem. 2011 Dec;401(10):3263-8. doi: 10.1007/s00216-011-5418-0. Epub 2011 Oct 4.
A method based on polarized attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy is proposed for determining the infrared dichroic absorption ratio of a single fiber from a sample deposited flat on a germanium crystal without the requirement of fiber orientation. The method shows its efficiency on cellulose fibers of paper and has been applied to protein fibers (type I collagen and β-amyloid) and polysaccharide fibers (cellulose and starch). The method gives access to the dichroic ratio of strong absorptions bands, which is not easily accessible with conventional absorption techniques. Then, the orientation of the molecular groups of organic fibers can be easily determined by polarized ATR-FTIR spectroscopy. By extension, this method will be useful to determine the molecular orientation of fibers in structured complex samples, such as biological tissues and plants. Spatially resolved information on the organization of the fiber network will be easily extracted by utilizing a focal plane array detector for imaging measurements.
提出了一种基于偏振衰减全反射傅里叶变换红外（ATR-FTIR）光谱的方法，用于确定无需纤维取向即可沉积在锗晶体上的平面样品中单根纤维的红外二向色吸收比。该方法在纸张的纤维素纤维上表现出了效率，并且已经应用于蛋白质纤维（I 型胶原蛋白和β-淀粉样蛋白）和多糖纤维（纤维素和淀粉）。该方法可获得强吸收带的二向色比值，这是常规吸收技术不易获得的。然后，通过偏振 ATR-FTIR 光谱可以轻松确定有机纤维分子基团的取向。通过扩展，该方法将有助于确定结构复杂样品（例如生物组织和植物）中纤维的分子取向。通过利用焦平面阵列探测器进行成像测量，可以轻松提取纤维网络组织的空间分辨信息。
