Wang Na, Wang Chan, Chen Hou, Bai Liangjiu, Wang Wenxiang, Yang Huawei, Wei Donglei, Yang Lixia
Key Laboratory of High Performance and Functional Polymer in the Universities of Shandong Province, Collaborative Innovation Center of Shandong Province for High Performance Fibers and Their Composites, School of Chemistry and Materials Science, Ludong University, Yantai 264025, China.
Anal Methods. 2020 Sep 28;12(36):4438-4446. doi: 10.1039/d0ay01151g. Epub 2020 Aug 28.
As robust functional polymers, polymer brush-based hybrid nanomaterials have potential application in the highly sensitive determination of tumor markers (TMs). Currently, there are plentiful reports on the polymerization methods of functional polymer brushes. Low ppm ATRP (activators (re)generated by electron transfer for atom transfer radical polymerization (A(R)GET ATRP), initiators for continuous activator regeneration atom transfer radical polymerization (ICAR ATRP) and electrochemically mediated atom transfer radical polymerization (eATRP)) is a facile and robust methodology with the advantages of simplicity, eco-friendliness and wide applicability to prepare well-defined polymeric materials. In this work, a controlled polymer brush-type functional nanoprobe is successfully fabricated by functional AGET ATRP and used as a sandwich-type electrochemical immunosensor for precise detection of TMs (alpha-fetoprotein, AFP). Using graphene oxide (GO) as an excellent conductive matrix, a GO-based poly-heterozygosis pyridine nanomaterial (GO@PHPY) is obtained by surface-initiated AGET ATRP and photocatalytic modification. The nanoprobe is assembled using GO@PHPY and a detection antibody (Ab2) to detect AFP, in which Cu(ii) serves as a signal label to coordinate with the pyridyl group. Under optimized conditions, the electrochemical sensor exhibits a good detection effect on AFP, with a detection range of 0.1 pg mL to 100 ng mL and a low detection limit of 0.08 pg mL. It is worth noting that the detection platform can be applied to the detection of real human serum samples. Thus, it is a desirable platform for AFP detection in clinical diagnosis and practical applications. Meanwhile, this work proves that designing and constructing functional polymer brushes is one of the most effective methods for developing new materials for analytical scientific applications.
作为性能稳健的功能聚合物，基于聚合物刷的杂化纳米材料在肿瘤标志物（TMs）的高灵敏度测定中具有潜在应用价值。目前，关于功能聚合物刷的聚合方法已有大量报道。低 ppm 的原子转移自由基聚合（ATRP）（电子转移再生催化剂原子转移自由基聚合（A(R)GET ATRP）、连续活化剂再生原子转移自由基聚合引发剂（ICAR ATRP）和电化学介导的原子转移自由基聚合（eATRP））是一种简便且稳健的方法，具有操作简单、环境友好以及广泛适用于制备结构明确的聚合物材料等优点。在本工作中，通过功能化的 AGET ATRP 成功制备了一种可控的聚合物刷型功能纳米探针，并将其用作夹心型电化学免疫传感器以精确检测肿瘤标志物（甲胎蛋白，AFP）。以氧化石墨烯（GO）作为优良的导电基质，通过表面引发的 AGET ATRP 和光催化改性获得了基于 GO 的聚杂合吡啶纳米材料（GO@PHPY）。该纳米探针由 GO@PHPY 和检测抗体（Ab2）组装而成用于检测 AFP，其中 Cu(ii)作为信号标记与吡啶基团配位。在优化条件下，该电化学传感器对 AFP 表现出良好的检测效果，检测范围为 0.1 pg mL 至 100 ng mL，检测限低至 0.08 pg mL。值得注意的是，该检测平台可应用于实际人血清样本的检测。因此，它是临床诊断和实际应用中 AFP 检测的理想平台。同时，这项工作证明了设计和构建功能聚合物刷是开发用于分析科学应用的新材料的最有效方法之一。
