Wang Na, Wang Jing, Zhao Xiaoqing, Chen Hou, Xu Hui, Bai Liangjiu, Wang Wenxiang, Yang Huawei, Wei Donglei, Yuan Baiqing
School of Chemistry and Materials Science, Ludong University, Key Laboratory of High Performance and Functional Polymer in the Universities of Shandong Province, Collaborative Innovation Center of Shandong Province for High Performance Fibers and Their Composites, Yantai, 264025, China.
School of Chemistry and Materials Science, Ludong University, Key Laboratory of High Performance and Functional Polymer in the Universities of Shandong Province, Collaborative Innovation Center of Shandong Province for High Performance Fibers and Their Composites, Yantai, 264025, China.
Talanta. 2021 May 1;226:122133. doi: 10.1016/j.talanta.2021.122133. Epub 2021 Jan 23.
A novel sandwich-type electrochemical immunosensor has been fabricated for simultaneous determination of three kinds of tumor markers (TMs). The signal-amplified platform for sensor was assisted by graphene oxide (GO) loaded with Poly(styrene-alt-maleic anhydride) (PSMA). Three TMs of prostate specific antigen (PSA), human a-fetoprotein (AFP) and carbohydrate antigen 125 (CA 125) were employed as model test molecules. This sandwich-type immunoassay could detect the three antigens in differential pulse voltammetry (DPV) scan well at the same time. Under the optimized conditions, the multiplexed immunosensor displayed a splendid linear response in the range of 1.13 pg mL - 113 ng mL for PSA, 0.35 pg mL - 35 ng mL for AFP and 0.025 U mL - 250 U mL for CA 125. The detection limit was 86 fg mL, 14 fg mL and 0.0019 U mL for PSA, AFP and CA 125, respectively. This strategy provides a simple and sensitive method for immunoassay for the identification and validation of specific early cancers.
一种新型夹心型电化学免疫传感器已被制备用于同时测定三种肿瘤标志物（TMs）。传感器的信号放大平台由负载有聚（苯乙烯-alt-马来酸酐）（PSMA）的氧化石墨烯（GO）辅助。以前列腺特异性抗原（PSA）、人甲胎蛋白（AFP）和糖类抗原125（CA 125）这三种肿瘤标志物作为模型测试分子。这种夹心型免疫测定法能够在差分脉冲伏安法（DPV）扫描中同时很好地检测这三种抗原。在优化条件下，该多重免疫传感器对PSA在1.13 pg mL - 113 ng mL范围内、对AFP在0.35 pg mL - 35 ng mL范围内以及对CA 125在0.025 U mL - 250 U mL范围内均呈现出良好的线性响应。PSA、AFP和CA 125的检测限分别为86 fg mL、14 fg mL和0.0019 U mL。该策略为免疫测定提供了一种简单且灵敏的方法，用于特定早期癌症的识别和验证。
