Api m 6 和 Api m 10 是蜜蜂毒液过敏患者的主要过敏原。

Api m 6 and Api m 10 as Major Allergens in Patients With Honeybee Venom Allergy.

机构信息

Hospital Universitario de Guadalajara, Guadalajara, Spain; Spanish Thematic Network and Co-operative Research Centre ARADyAL, Center RD16/0006/0023.

Diater Laboratorios S.A., Leganés, Madrid, Spain.

出版信息

J Investig Allergol Clin Immunol. 2022 Apr 19;32(2):116-123. doi: 10.18176/jiaci.0639. Epub 2020 Aug 26.

DOI:10.18176/jiaci.0639

PMID:32856591

Abstract

BACKGROUND

Component-resolved diagnosis plays a key role in the diagnosis and treatment of honeybee venom allergy (HVA). Our aim was to study whether any of the allergens not included in the usual diagnostic platforms are relevant in our population.

MATERIAL AND METHODS

The allergenic sensitization profile of Spanish patients who experienced a systemic reaction after a honeybee sting and were diagnosed with HVA was studied by immunoblotting based on raw autochthonous Apis mellifera venom characterized using SDS-PAGE and mass spectrometry and a commercial assay (ImmunoCAP).

RESULTS

Allergens in the International Union of Immunological Societies database were detected in the raw A mellifera venom extract used, except Api m 12. Sera from 51 patients with a median (IQR) age of 46.2 years (35.6-54.6) were analyzed. ImmunoCAP revealed Api m 1 and Api m 10 to be major allergens (88.2% and 74.5%, respectively). Moreover, Api m 6 (85.4%) was detected by immunoblotting.

CONCLUSION

Api m 1, Api m 6, and Api m 10 are major A mellifera venom allergens in our population.

摘要

背景

组分诊断在诊断和治疗蜂毒过敏（HVA）中起着关键作用。我们的目的是研究在我们的人群中，是否有任何未包含在常规诊断平台中的过敏原具有相关性。

材料和方法

通过免疫印迹法研究了因被蜜蜂蛰伤而出现全身性反应并被诊断为 HVA 的西班牙患者的过敏原致敏谱，该方法基于使用 SDS-PAGE 和质谱法对原始的土生土长的 Apis mellifera 毒液进行特征分析，以及使用商业检测（ImmunoCAP）。

结果

除了 Api m 12 之外，在使用的原始 A mellifera 毒液提取物中检测到了国际免疫学会联合会数据库中的过敏原。对 51 名中位（IQR）年龄为 46.2 岁（35.6-54.6）的患者的血清进行了分析。ImmunoCAP 显示 Api m 1 和 Api m 10 为主要过敏原（分别为 88.2%和 74.5%）。此外，免疫印迹法检测到 Api m 6（85.4%）。