College of Food Science and Engineering, Northwest A&F University, Yangling 712100, Shaanxi, China.
J Agric Food Chem. 2020 Oct 14;68(41):11578-11585. doi: 10.1021/acs.jafc.0c03832. Epub 2020 Sep 9.
In view of the potential harm caused by illegal feeding of clenbuterol (CLB) in the livestock industry, herein, a novel ratiometric fluorescent probe based on graphene quantum dots (GQDs)@[Ru(bpy)] was elaborately constructed for CLB detection. In this probe, GQDs acted as response signals, and their fluorescence was remarkably quenched by CLB through the diazotization-coupling reaction. As for [Ru(bpy)] as a reference signal, its fluorescence was hardly affected. The intensity ratio of two fluorophores showed good linearity with CLB concentration in the range of 0.05-40 μM, accompanied by visualization of fluorescence variation from yellow to red. The detection limit was as low as 0.029 μM. Particularly, the probe was successfully used to detect CLB in pork and beef samples with satisfactory recoveries. To our knowledge, this is the first report on a ratiometric fluorescent probe for the detection of CLB, which possesses broad application prospects in food safety risk monitoring.
鉴于畜牧业中非法添加克伦特罗（CLB）带来的潜在危害，本研究设计了一种基于石墨烯量子点（GQDs）@[Ru(bpy)]的比率型荧光探针，用于 CLB 的检测。在该探针中，GQDs 作为响应信号，其荧光通过重氮化偶联反应被 CLB 显著猝灭。而 [Ru(bpy)] 作为参比信号，其荧光几乎不受影响。两种荧光团的强度比与 CLB 浓度在 0.05-40 μM 范围内呈良好的线性关系，同时荧光颜色从黄色变为红色，实现了可视化变化。检测限低至 0.029 μM。特别地，该探针成功用于猪肉和牛肉样品中 CLB 的检测，回收率令人满意。据我们所知，这是首例用于检测 CLB 的比率型荧光探针，在食品安全风险监测方面具有广阔的应用前景。
