Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education), College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha, 410081, People's Republic of China.
J Fluoresc. 2017 Sep;27(5):1847-1853. doi: 10.1007/s10895-017-2122-2. Epub 2017 Jun 20.
Clenbuterol (CLB), a member of β-agonist family, has now been a serious threat to human health due to its illegal usage in the animal feed. In this paper, we designed a fluorescence resonance energy transfer (FRET) system consisting of carbon dots (C-dots) and gold nanoparticles (AuNPs) for recovered fluorescence detecting of CLB. In the presence of CLB, CLB molecules can interact with AuNPs via Au-N bonds, preventing the interaction of C-dots and AuNPs, which induced the recover of the fluorescent intensity. Under the optimal conditions, the limit of detection for CLB was 3 nM, with a wide concentration linear range of 8-200 nM (S/N = 3). Meanwhile, the proposed method was successfully applied to detect CLB in pork samples, illustrating it could be used as a reliable, rapid, and cost-effective method for the determination of CLB residues in pork samples.
克仑特罗（CLB）是β-激动剂家族的一员，由于其在动物饲料中的非法使用，现已对人类健康构成严重威胁。在本文中，我们设计了一个由碳点（C-dots）和金纳米粒子（AuNPs）组成的荧光共振能量转移（FRET）系统，用于回收荧光检测 CLB。在 CLB 的存在下，CLB 分子可以通过 Au-N 键与 AuNPs 相互作用，阻止 C-dots 和 AuNPs 的相互作用，从而导致荧光强度的恢复。在最佳条件下，CLB 的检测限为 3 nM，具有 8-200 nM 的宽浓度线性范围（S/N = 3）。同时，该方法成功应用于猪肉样品中 CLB 的检测，表明它可以作为一种可靠、快速且具有成本效益的方法，用于测定猪肉样品中的 CLB 残留量。
