State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China; Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, Xiamen University, Xiamen 361102, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, China; Fujian Key Laboratory of Genetics and Breeding of Marine Organisms, Xiamen University, Xiamen 361102, China; College of Ocean and Earth Sciences, Xiamen University, Xiamen, China.
J Photochem Photobiol B. 2020 Oct;211:111994. doi: 10.1016/j.jphotobiol.2020.111994. Epub 2020 Aug 12.
Circadian rhythm is the most important and universal biological rhythm in marine organisms. In this research, the movement behaviour of abalone (Haliotis discus hannai) was continuously monitored under a light cycle of 12 L:12D. It was found that the cumulative movement distance and cumulative movement time of abalone reached was highest from 00:00-03:00 h. The minimum values of maximum movement velocity occurred between 21:00-00:00 h, and a significant circadian cosine rhythm was exhibited during these periods (P < 0.05). Metabolomic analysis of cerebral ganglions of abalone was conducted at 06:00 h (6 M), 14:00 h (14 M), and 22:00 h (22 M) and 380, 385, and 315 metabolites with significant differences were identified in 6 M vs 14 M, 14 M vs 22 M, and 6 M vs 22 M, respectively (P < 0.05). With the alternation of day and night, the expression levels of phosphatidylcholine, 5-HT, N-acetyl-5-hydroxytryptamine, indole-3-acetaldehyde, hypoxanthine, and deoxyinosine declined significantly, while those of Lysophosphatidylcholines (lysoPC) (20: 5 (5Z, 8Z, 11Z, 14Z, 17Z)), lysoPC (22: 4 (7Z, 10Z, 13Z, 16Z)), lysoPC (16: 1 (9Z) / 0: 0), phosphatidylethanolamine (PE) (18: 1 (11Z) 22: 2 (13Z, 16Z)), and guanosine 5'-phosphate rose significantly. These 11 metabolites can be used as differential metabolic markers. These findings not only quantitatively describe the circadian movement behaviours of abalone, but also provide an initial analysis of the circadian mechanism of the physiological metabolic conversion of abalone, which in turn provides guidelines for light control and feeding strategy for use in aquaculture production.
昼夜节律是海洋生物中最重要和最普遍的生物节律。在这项研究中，连续监测了鲍鱼（Haliotis discus hannai）在 12L:12D 光周期下的运动行为。结果发现，鲍鱼的累积运动距离和累积运动时间在 00:00-03:00 时达到最高。最大运动速度的最小值出现在 21:00-00:00 之间，并且在此期间表现出明显的昼夜余弦节律（P<0.05）。在 06:00 时（6M）、14:00 时（14M）和 22:00 时（22M）对鲍鱼的脑神经节进行了代谢组学分析，在 6M 与 14M、14M 与 22M 以及 6M 与 22M 之间分别鉴定出 380、385 和 315 个具有显著差异的代谢物（P<0.05）。随着昼夜的交替，磷脂酰胆碱、5-HT、N-乙酰-5-羟色胺、吲哚-3-乙醛、次黄嘌呤和脱氧肌苷的表达水平显著下降，而溶血磷脂酰胆碱（lysoPC）（20:5（5Z、8Z、11Z、14Z、17Z））、lysoPC（22:4（7Z、10Z、13Z、16Z））、lysoPC（16:1（9Z）/0:0））、磷脂酰乙醇胺（PE）（18:1（11Z）22:2（13Z、16Z））和鸟苷 5'-磷酸的表达水平显著升高。这 11 种代谢物可以作为差异代谢标志物。这些发现不仅定量描述了鲍鱼的昼夜运动行为，还初步分析了鲍鱼生理代谢转化的昼夜机制，为水产养殖生产中的光照控制和投喂策略提供了指导。
