Di G, Luo X, You W, Zhao J, Kong X, Ke C
1] State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, PR China [2] College of Fisheries, Henan Normal University, Xinxiang, PR China.
State Key Laboratory of Marine Environmental Science, College of Ocean and Earth Sciences, Xiamen University, Xiamen, PR China.
Heredity (Edinb). 2015 Jun;114(6):564-74. doi: 10.1038/hdy.2014.124. Epub 2015 Feb 11.
To understand the potential molecular mechanism of heterosis, protein expression patterns were compared from hybrids of Haliotis gigantea (G) and Haliotis discus hannai (D) using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight/time-of-flight analyses. Expression differences were observed in muscle samples from the four groups with 673±21.0 stained spots for H. discus hannai ♀ × H. discus hannai ♂ (DD), 692±25.6 for H. gigantea ♀ × H. gigantea ♂ (GG), 679±16.2 for H. discus hannai ♀ × H. gigantea ♂ (DG) (F1 hybrid) and 700±19 for H. gigantea ♀ × H. discus hannai ♂ (GD) (F1 hybrid). Different 2-DE image muscle protein spots had a mirrored relationship between purebreds and the F1 hybrid, suggesting that all stained spots in F1 hybrid muscle were on 2-DEs from parents. DD and DG clustered together first, and then clustered with GD, whereas the distance of DD and GG was maximal according to hierarchical cluster analysis. We identified 136 differentially expressed protein spots involved in major biological processes, including energy metabolism and stress response. Most energy metabolism proteins were additive, and stress-induced proteins displayed additivity or over-dominance. In these 136 identified protein spots, hybrid offspring with additivity or over-dominance accounted for 68.38%. Data show that a proteomic approach can provide functional prediction of abalone interspecific hybridization.
为了解杂种优势的潜在分子机制，利用二维凝胶电泳（2-DE）和基质辅助激光解吸/电离飞行时间/飞行时间分析，比较了皱纹盘鲍（G）和盘鲍（D）杂交种的蛋白质表达模式。在四组肌肉样本中观察到表达差异，皱纹盘鲍♀×皱纹盘鲍♂（DD）有673±21.0个染色点，皱纹盘鲍♀×皱纹盘鲍♂（GG）有692±25.6个染色点，皱纹盘鲍♀×皱纹盘鲍♂（DG）（F1杂种）有679±16.2个染色点，皱纹盘鲍♀×皱纹盘鲍♂（GD）（F1杂种）有700±19个染色点。不同的2-DE图像肌肉蛋白质点在纯种和F1杂种之间呈镜像关系，表明F1杂种肌肉中的所有染色点都来自亲本的2-DE。根据层次聚类分析，DD和DG首先聚类在一起，然后与GD聚类，而DD和GG之间的距离最大。我们鉴定出136个参与主要生物学过程的差异表达蛋白质点，包括能量代谢和应激反应。大多数能量代谢蛋白具有加性，应激诱导蛋白表现出加性或超显性。在这136个鉴定出的蛋白质点中，具有加性或超显性的杂种后代占68.38%。数据表明，蛋白质组学方法可以为鲍鱼种间杂交提供功能预测。
