Valadares Rafael B S, Perotto Silvia, Lucheta Adriano R, Santos Eder C, Oliveira Renato M, Lambais Marcio R
Escola Superior de Agricultura "Luiz de Queiroz", Depto de Ciência do Solo, Universidade de São Paulo, Av. Pádua Dias 11, Piracicaba 13418-900, Brazil.
Instituto Tecnológico Vale. Rua Boaventura da Silva 955, Belém 66050-000, Brazil.
J Fungi (Basel). 2020 Aug 26;6(3):148. doi: 10.3390/jof6030148.
Orchids form endomycorrhizal associations with fungi mainly belonging to basidiomycetes. The molecular events taking place in orchid mycorrhiza are poorly understood, although the cellular changes necessary to accommodate the fungus and to control nutrient exchanges imply a modulation of gene expression. Here, we used proteomics and transcriptomics to identify changes in the steady-state levels of proteins and transcripts in the roots of the green terrestrial orchid . When mycorrhizal and non-mycorrhizal roots from the same individuals were compared, 94 proteins showed differential accumulation using the label-free protein quantitation approach, 86 using isobaric tagging and 60 using 2D-differential electrophoresis. After assembly of transcriptomic data, 11,179 plant transcripts were found to be differentially expressed, and 2175 were successfully annotated. The annotated plant transcripts allowed the identification of up- and down-regulated metabolic pathways. Overall, proteomics and transcriptomics revealed, in mycorrhizal roots, increased levels of transcription factors and nutrient transporters, as well as ethylene-related proteins. The expression pattern of proteins and transcripts involved in plant defense responses suggested that plant defense was reduced in mycorrhizal roots sampled in nature. These results expand our current knowledge towards a better understanding of the orchid mycorrhizal symbiosis in adult plants under natural conditions.
兰花与主要属于担子菌的真菌形成内生菌根共生关系。尽管容纳真菌和控制养分交换所需的细胞变化意味着基因表达的调节，但兰花菌根中发生的分子事件仍知之甚少。在这里，我们使用蛋白质组学和转录组学来鉴定绿色地生兰花根中蛋白质和转录本稳态水平的变化。当比较来自同一植株的菌根根和非菌根根时，采用无标记蛋白质定量方法有94种蛋白质表现出差异积累，采用等压标记法有86种，采用二维差异电泳法有60种。在转录组数据组装后，发现11179个植物转录本差异表达，其中2175个成功注释。注释后的植物转录本有助于识别上调和下调的代谢途径。总体而言，蛋白质组学和转录组学显示，在菌根根中，转录因子、养分转运蛋白以及乙烯相关蛋白的水平升高。参与植物防御反应的蛋白质和转录本的表达模式表明，在自然条件下采集的菌根根中植物防御能力降低。这些结果扩展了我们目前的知识，有助于更好地理解自然条件下成年植物中的兰花菌根共生关系。
