Valadares Rafael B S, Marroni Fabio, Sillo Fabiano, Oliveira Renato R M, Balestrini Raffaella, Perotto Silvia
Instituto Tecnológico Vale, Rua Boaventura da Silva 955, 66050-000 Belém, Pará, Brazil.
Dipartimento di Scienze Agroalimentari, Ambientali e Animali, Università di Udine, Via delle Scienze, I-33100 Udine, Italy.
Plants (Basel). 2021 Jan 28;10(2):251. doi: 10.3390/plants10020251.
The study of orchid mycorrhizal interactions is particularly complex because of the peculiar life cycle of these plants and their diverse trophic strategies. Here, transcriptomics has been applied to investigate gene expression in the mycorrhizal roots of , a terrestrial mixotrophic orchid that associates with ectomycorrhizal fungi in the genus . Our results provide new insights into the mechanisms underlying plant-fungus interactions in adult orchids in nature and in particular into the plant responses to the mycorrhizal symbiont(s) in the roots of mixotrophic orchids. Our results indicate that amino acids may represent the main nitrogen source in mycorrhizal roots of , as already suggested for orchid protocorms and other orchid species. The upregulation, in mycorrhizal roots, of some symbiotic molecular marker genes identified in mycorrhizal roots from other orchids as well as in arbuscular mycorrhiza, may mirror a common core of plant genes involved in endomycorrhizal symbioses. Further efforts will be required to understand whether the specificities of orchid mycorrhiza depend on fine-tuned regulation of these common components, or whether specific additional genes are involved.
由于这些植物独特的生命周期及其多样的营养策略，对兰花菌根相互作用的研究格外复杂。在此，转录组学已被应用于研究一种与 属外生菌根真菌共生的陆生兼养型兰花的菌根根中的基因表达。我们的研究结果为自然环境中成年兰花植物与真菌相互作用的潜在机制提供了新见解，特别是关于兼养型兰花根中植物对菌根共生体的反应。我们的结果表明，氨基酸可能是 菌根根中的主要氮源，正如之前对兰花原球茎和其他兰花物种所提出的那样。在菌根根中，一些在其他兰花的菌根根以及丛枝菌根中鉴定出的共生分子标记基因的上调，可能反映了参与内生菌根共生的植物基因的共同核心。还需要进一步努力来了解兰花菌根的特异性是取决于这些共同成分的精细调控，还是涉及特定的其他基因。
