Metabolic and Structural Biology Department, CSIR-Central Institute of Medicinal and Aromatic Plants (CSIR-CIMAP), Lucknow, 226015, India.
Department of Biochemistry, Central University of Haryana, Mahendergarh, Haryana, 123029, India.
Mol Biol Rep. 2020 Sep;47(9):6587-6598. doi: 10.1007/s11033-020-05710-1. Epub 2020 Aug 28.
Genus Ocimum is known to have species possessing important therapeutic essential oil. The major phytoconstituents of essential oil in Ocimum species are phenylpropanoids and terpenoids. The essential oil is accumulated in the trichomes; the specialized structures predominantly found on leaves and other tissues. The development of trichome is integrated with development of plant and leaf and also tightly coordinated with the primary and secondary metabolic pathways producing essential oil constituents. In continuation to our studies on elucidating/understanding the mechanism of biosynthesis of essential oil pathways in Ocimum species, we have performed comparative transcriptome analysis to investigate the role of trichome-related gene expression in the regulation of biosynthetic pathways of essential oil. The essential oil biogenesis is tightly integrated with primary metabolic activities, the analysis for the expression pattern of genes related to primary metabolism and its relationship with secondary metabolism was evaluated in comparative manner. Physiological parameters in relation to primary metabolism such as photosynthetic pigment content, soluble sugar content, and invertase enzymes along with morphological parameters were analysed in O. basilicum and O. sanctum. Differential expression profiling uncovered about 8116 and 2810 differentially expressed transcripts in O. basilicum and O. sanctum, respectively. Enrichment of differentially expressed genes were analysed in relation to metabolic pathways, primary metabolism and secondary metabolism. Trichome related genes identified from the Ocimum species vis-à-vis their expression profiles suggested higher expression in O. basilicum. The findings in this study provide interesting insights into the role of trichome-related transcripts in relation to essential oil content in Ocimum species. The study is valuable as this is the first study on revealing the transcripts and their role in trichome development and essential oil biogenesis in two major species of Ocimum.
罗勒属被认为拥有具有重要治疗作用的芳香油。罗勒属植物精油的主要植物化学成分是苯丙烷类和萜烯类。精油积累在毛状体中；毛状体是主要存在于叶片和其他组织中的特化结构。毛状体的发育与植物和叶片的发育是整合在一起的，并且与产生精油成分的初级和次级代谢途径紧密协调。在继续我们对阐明/理解罗勒属植物精油生物合成途径的研究中，我们进行了比较转录组分析，以研究毛状体相关基因表达在调节精油生物合成途径中的作用。精油生物合成与初级代谢活动紧密结合，因此评估了与初级代谢相关的基因表达模式及其与次级代谢的关系。与初级代谢有关的生理参数，如光合色素含量、可溶性糖含量和转化酶酶，以及形态参数，在罗勒和神圣罗勒中进行了分析。差异表达谱分析在罗勒和神圣罗勒中分别发现了约 8116 个和 2810 个差异表达转录本。对差异表达基因进行了与代谢途径、初级代谢和次级代谢相关的富集分析。从罗勒属物种中鉴定的毛状体相关基因及其表达谱表明，在罗勒中表达更高。这项研究的发现提供了有关毛状体相关转录物在罗勒属物种精油含量中的作用的有趣见解。这项研究很有价值，因为这是首次揭示两个主要罗勒属物种中毛状体发育和精油生物合成相关转录物及其作用的研究。
