Department of Pathogenic Biology, Chengde Medical University, Chengde, 067000, Hebei, China.
Department of Pathogenic Biology, Chengde Medical University, Chengde, 067000, Hebei, China.
Exp Parasitol. 2020 Nov;218:107983. doi: 10.1016/j.exppara.2020.107983. Epub 2020 Aug 28.
Trichinella spiralis (T. spiralis) muscle larvae (ML) excretory/secretory products (ESPs) are antitumor substances extracted from the culture medium of T. spiralis ML. The ESPs inhibit tumor growth and induce tumor cell apoptosis. To explore the effects of these products on the non-small-cell lung cancer (NSCLC) line A549, logarithmically growing A549 cells were co-cultured with different concentrations of T. spiralis ML ESPs for 24, 36 and 48 h. Our results showed that T. spiralis ML ESPs significantly inhibited A549 cells proliferation, which was dose-and time-dependent. To evaluate the inhibition by T. spiralis ML ESPs of the growth of A549 cells, we assayed their apoptosis and cell-cycle distribution by flow cytometry (FCM). To determine whether ESPs induced apoptosis of A549 cells via the mitochondrial pathway, we evaluated the levels of mitochondrion-related factors by Western blotting. The FCM indicated a clear trend toward apoptosis of A549 cells co-cultured with ESPs for 24 h. The cells were blocked in S-phase. Western blotting revealed that the expression levels of the genes encoding Bax, caspase-3, and caspase-9 increased (compared to a control group), and the Bcl-2 gene expression level decreased. Our results suggest that T. spiralis ML ESPs induce apoptosis of the NSCLC line A549 via the mitochondrial pathway; the cells become arrested in S-phase. This may explain the antineoplastic activity of T. spiralis ML ESPs.
旋毛虫肌肉幼虫（ML）排泄/分泌产物（ESPs）是从旋毛虫 ML 培养物中提取的抗肿瘤物质。ESPs 抑制肿瘤生长并诱导肿瘤细胞凋亡。为了研究这些产物对非小细胞肺癌（NSCLC）细胞系 A549 的影响，对数生长期的 A549 细胞与不同浓度的旋毛虫 ML ESP 共同培养 24、36 和 48 小时。结果表明，旋毛虫 ML ESP 显著抑制 A549 细胞增殖，呈剂量和时间依赖性。为了评估旋毛虫 ML ESP 对 A549 细胞生长的抑制作用，我们通过流式细胞术（FCM）检测了它们的凋亡和细胞周期分布。为了确定 ESP 是否通过线粒体途径诱导 A549 细胞凋亡，我们通过 Western blot 评估了线粒体相关因子的水平。FCM 显示出与 ESP 共培养 24 小时的 A549 细胞凋亡的明显趋势。细胞被阻滞在 S 期。Western blot 显示，编码 Bax、caspase-3 和 caspase-9 的基因表达水平增加（与对照组相比），Bcl-2 基因表达水平降低。我们的结果表明，旋毛虫 ML ESP 通过线粒体途径诱导 NSCLC 细胞系 A549 凋亡；细胞被阻滞在 S 期。这可能解释了旋毛虫 ML ESP 的抗肿瘤活性。
