抗骨肉瘤血清对骨肉瘤与旋毛虫交叉抗原 TPD52 的抗骨肉瘤作用。
Anti-osteosarcoma effect of antiserum against cross antigen TPD52 between osteosarcoma and Trichinella spiralis.
机构信息
Key Laboratory of Zoonosis Research By Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, Jilin University, Changchun, 130062, China.
出版信息
Parasit Vectors. 2021 Sep 26;14(1):498. doi: 10.1186/s13071-021-05008-6.
BACKGROUND
Trichinella spiralis (T. spiralis) is a parasite occurring worldwide that has been proven to have antitumour ability. However, studies on the antitumour effects of cross antigens between the tumour and T. spiralis or antibodies against cross antigens between tumours and T. spiralis are rare.
METHODS
To study the role of cross antigens between osteosarcoma and T. spiralis, we first screened the cDNA expression library of T. spiralis muscle larvae to obtain the cross antigen gene tumour protein D52 (TPD52), and prepared fusion protein TPD52 and its antiserum. The anti-osteosarcoma effect of the anti-TPD52 antiserum was studied using cell proliferation and cytotoxicity assays as well as in vivo animal models; preliminary data on the mechanism were obtained using western blot and immunohistochemistry analyses.
RESULTS
Our results indicated that TPD52 was mainly localized in the cytoplasm of MG-63 cells. Anti-TPD52 antiserum inhibited the proliferation of MG-63 cells and the growth of osteosarcoma in a dose-dependent manner. The tumour inhibition rate in the 100 μg treatment group was 61.95%. Enzyme-linked immunosorbent assay showed that injection of anti-TPD52 antiserum increased the serum levels of IFN-γ, TNF-α, and IL-12 in nude mice. Haematoxylin and eosin staining showed that anti-TPD52 antiserum did not cause significant pathological damage. Apoptosis of osteosarcoma cells was induced by anti-TPD52 antiserum in vivo and in vitro.
CONCLUSIONS
Anti-TPD52 antiserum exerts an anti-osteosarcoma effect by inducing apoptosis without causing histopathological damage.
背景
旋毛虫（T. spiralis）是一种世界性寄生虫，已被证明具有抗肿瘤能力。然而，关于肿瘤与旋毛虫之间的交叉抗原或肿瘤与旋毛虫之间的抗体的抗肿瘤作用的研究很少。
方法
为了研究骨肉瘤与旋毛虫之间的交叉抗原的作用，我们首先筛选旋毛虫肌肉幼虫的 cDNA 表达文库，获得交叉抗原基因肿瘤蛋白 D52（TPD52），并制备融合蛋白 TPD52 及其抗血清。使用细胞增殖和细胞毒性测定以及体内动物模型研究抗 TPD52 抗血清对骨肉瘤的抗作用；使用 Western blot 和免疫组织化学分析获得初步的机制数据。
结果
我们的结果表明 TPD52 主要定位于 MG-63 细胞的细胞质中。抗 TPD52 抗血清以剂量依赖的方式抑制 MG-63 细胞的增殖和骨肉瘤的生长。在 100μg 处理组中，肿瘤抑制率为 61.95%。酶联免疫吸附试验显示，注射抗 TPD52 抗血清可增加裸鼠血清中 IFN-γ、TNF-α 和 IL-12 的水平。苏木精和伊红染色显示，抗 TPD52 抗血清未引起明显的病理损伤。抗 TPD52 抗血清在体内和体外诱导骨肉瘤细胞凋亡。
结论
抗 TPD52 抗血清通过诱导凋亡而不引起组织病理学损伤发挥抗骨肉瘤作用。
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