Yun Li, Zhaoming Zheng, Jun Li, Baihang Zhao, Wei Bian, Yanzhuo Zhang, Xiujie Wang
The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing 1000124, China E-mail:
Water Sci Technol. 2016 Dec;74(11):2697-2707. doi: 10.2166/wst.2016.449.
The present study reports the inhibition kinetics and granular sludge in an anaerobic ammonium oxidation (ANAMMOX) - up-flow anaerobic sludge blanket reactor fed with diluted mature landfill leachate. The activity of ANAMMOX bacteria was inhibited by addition of mature landfill leachate, but gradually adapted to the leachate. The system achieved efficient nitrogen removal during 65-75 d and the average removal efficiencies for NH-N, NO-N and total nitrogen (TN) were 96%, 95% and 87%, respectively. ANAMMOX was the main pathway of nitrogen removal in the system, and heterotrophic denitrification occurred simultaneously. In addition, aerobic ammonia oxidation and aerobic nitrite oxidation were active in this system. Inhibition kinetic experiments showed that the NH-N and NO-N inhibition concentration threshold of ANAMMOX were 489.03 mg/L and 192.36 mg/L, respectively. ANAMMOX was significantly inhibited by mature landfill leachate, and was completely inhibited when the leachate concentration was 1,450.69 mg/L (calculated in chemical oxygen demand). Thus, the inhibition concentration of substrate and landfill leachate should be considered when applying the ANAMMOX process to landfill leachate. The color of granular sludge ANAMMOX changed from brick-red into a reddish-brown. The particle size increased from small to large, with evident granulation of the ANAMMOX sludge.
本研究报告了在厌氧氨氧化（ANAMMOX）-上流式厌氧污泥床反应器中，以稀释的成熟垃圾渗滤液为进水时的抑制动力学和颗粒污泥情况。添加成熟垃圾渗滤液会抑制ANAMMOX细菌的活性，但该系统会逐渐适应渗滤液。在65 - 75天内系统实现了高效的氮去除，NH-N、NO-N和总氮（TN）的平均去除效率分别为96%、95%和87%。ANAMMOX是该系统中氮去除的主要途径，同时也发生了异养反硝化作用。此外，好氧氨氧化和好氧亚硝酸盐氧化在该系统中也很活跃。抑制动力学实验表明，ANAMMOX对NH-N和NO-N的抑制浓度阈值分别为489.03 mg/L和192.36 mg/L。成熟垃圾渗滤液对ANAMMOX有显著抑制作用，当渗滤液浓度为1450.69 mg/L（以化学需氧量计）时，ANAMMOX被完全抑制。因此，将ANAMMOX工艺应用于垃圾渗滤液时，应考虑底物和垃圾渗滤液的抑制浓度。ANAMMOX颗粒污泥的颜色从砖红色变为红棕色。粒径由小变大，ANAMMOX污泥的颗粒化明显。
