Institut Universitaire de Cardiologie et de Pneumologie de Québec/Québec Heart & Lung Institute, Laval University, Québec City, Québec, Canada.
Institut Universitaire de Cardiologie et de Pneumologie de Québec/Québec Heart & Lung Institute, Laval University, Québec City, Québec, Canada.
Exp Gerontol. 2020 Oct 15;140:111075. doi: 10.1016/j.exger.2020.111075. Epub 2020 Aug 27.
LDLr/ApoB/IGF-II mice are used as a calcific aortic valve disease (CAVD) model. However, normal aortic valve hemodynamics i.e. remotely from CAVD onset and the sex-related differences are poorly known.
Four groups of mice, intact males (IM, n = 49) and females (IF, n = 50), castrated males (CxM, n = 79) and ovariectomized females (OxF: 73), underwent a Doppler-echocardiography at 12 weeks of age. Gonadectomy was performed at 8 weeks. Aortic valve assessment using effective orifice area (EOA, using the continuity equation) and peak aortic transvalvular velocity (V) was feasible in 89% of the mice with good to excellent reliability (intraclass correlation coefficients ranging from 0.90 to 0.98, p < 0.001). Mean V was 104 ± 17 cm/s and mean EOA was 1.1810 ± 0.2210 cm. EOA indexed to body surface area was 1.5 ± 0.3 cm/m. The 95th percentile of Vpeak was 132 cm/s and the 5th percentile of indexed EOA was 1.0 cm/m. Interestingly, IM had the highest V (114 ± 14 cm/s) vs each of the other groups (CxM: 106 ± 19 cm/s, OxF: 97 ± 13 cm/s and IF: 96 ± 12 cm/s, ANOVA and corrected p < 0.001). This was mostly explained by a higher stroke volume (ANOVA and corrected p < 0.001) in IM compared to other groups. There were no major sex-differences in ventricular systolic function parameters.
In LDLr/ApoB/IGF-II CAVD mice model, an aortic EOA <0.8*10 cm (or indexed EOA <1.0cm/m), and a peak aortic valve velocity > 132 cm/s may be proposed as thresholds to define CAVD. Intact male mice appear to have higher velocities.
LDLr/ApoB/IGF-II 小鼠被用作钙化性主动脉瓣疾病 (CAVD) 模型。然而，正常主动脉瓣血流动力学，即远离 CAVD 发病和性别相关差异的情况知之甚少。
四组小鼠，完整雄性（IM，n=49）和雌性（IF，n=50）、去势雄性（CxM，n=79）和去卵巢雌性（OxF：n=73），在 12 周龄时进行多普勒超声心动图检查。性腺切除术在 8 周龄进行。使用有效瓣口面积（EOA，使用连续方程）和峰值主动脉瓣跨瓣速度（V）评估主动脉瓣在 89%的小鼠中是可行的，具有良好到极好的可靠性（组内相关系数范围为 0.90 至 0.98，p<0.001）。平均 V 为 104±17cm/s，平均 EOA 为 1.1810±0.2210cm。体表面积指数的 EOA 为 1.5±0.3cm/m。Vpeak 的第 95 百分位数为 132cm/s，指数化 EOA 的第 5 百分位数为 1.0cm/m。有趣的是，IM 的 V 最高（114±14cm/s），高于其他各组（CxM：106±19cm/s、OxF：97±13cm/s 和 IF：96±12cm/s，ANOVA 和校正后的 p<0.001）。这主要是由于 IM 的每搏量（ANOVA 和校正后的 p<0.001）高于其他组。心室收缩功能参数在性别间无明显差异。
在 LDLr/ApoB/IGF-II CAVD 小鼠模型中，主动脉瓣 EOA<0.8*10cm（或指数化 EOA<1.0cm/m）和峰值主动脉瓣速度>132cm/s 可能被提议作为定义 CAVD 的阈值。完整雄性小鼠似乎具有更高的速度。
