Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China; Research Center for Biomedical and Health Science, School of Life and Health, Anhui Science & Technology University, Fengyang 233100, PR China.
Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, PR China.
Food Chem. 2021 Mar 1;339:127891. doi: 10.1016/j.foodchem.2020.127891. Epub 2020 Aug 20.
We propose a visual strategy for simultaneous detection of multiple adulterated components in beef by integration of multiple polymerase chain reaction (mPCR) with the lateral flow strip (LFS). The primer sets for adulterated components are uniquely designed with different nucleic acid tags (NAT), enabling the amplicons with specific wobbled sequences at two opposite ends. The wobbled sequences will precisely hybridize with the pre-immobilized capture probes on T lines (T1, T2 and T3) and C line, contributing to the coloration of LFS. Taking advantages of extraordinary amplification efficiency of PCR and simplicity of LFS, common adulterated components including chicken, duck and pork can be easily detected with LOD as low as 0.01% (wt%), which is comparable to that of quantitative real-time polymerase chain reaction (qPCR) but with more simplified operations and reduced costs. The method can be extended to identification of other components by replacing the functional primer set. This method can be a useful candidate for meat quality control at the resource-limited setups.
我们提出了一种通过将多重聚合酶链反应（mPCR）与横向流动条（LFS）相结合来同时检测牛肉中多种掺假成分的视觉策略。掺假成分的引物组采用独特的核酸标签（NAT）设计，使两端具有特定摆动序列的扩增子。摆动序列将与 T 线（T1、T2 和 T3）和 C 线上预先固定的捕获探针精确杂交，导致 LFS 显色。利用 PCR 的非凡扩增效率和 LFS 的简单性，包括鸡肉、鸭肉和猪肉在内的常见掺假成分可以很容易地检测到，LOD 低至 0.01%（wt%），与定量实时聚合酶链反应（qPCR）相当，但操作更简单，成本更低。通过更换功能引物组，该方法可以扩展到其他成分的鉴定。该方法可以成为资源有限环境下肉类质量控制的有用候选方法。
