Wang Hanling, Meng Xianzhuo, Yao Li, Wu Qian, Yao Bangben, Chen Zhaoran, Xu Jianguo, Chen Wei
Engineering Research Center of Bio-process, MOE, School of Food and Biological Engineering, Hefei University of Technology, Hefei 230009, China.
School of Food Science and Bioengineering, Changsha University of Science & Technology, Changsha 410114, China.
Food Chem (Oxf). 2023 Aug 12;7:100180. doi: 10.1016/j.fochms.2023.100180. eCollection 2023 Dec 30.
Meat adulteration-based food fraud has recently become one of the global major economical, illegal, religious, and public health concerns. In this work, we developed a microarray chip polymerase chain reaction (PCR)-directed microfluidic lateral flow strip (LFS) device that facilitates the accurate and simultaneous identification of beef adulterated with chicken, duck, and pork, especially in processed beef products. To realize this goal, four pairs of amplification primers were designed and applied for specifically amplifying genomic DNA extracted from mixed meat powders in microarray chip. With the prominent advantage of this device lies in the flexible combination and integration of sample loading, detection, and reporting in microstructures, all the DNA amplicons can be individually visualized on the LFS unit, leading to the appearance of test lines (T line, T line, T line, or T line) as well as the control line (C line) for the species identification and quantification in beef products. Based on this new method, the adulterants were successfully distinguished and identified in mixtures down to 0.01% (wt.%) while the carryover aerogel contamination in routine molecular diagnostic laboratories was effectively avoided. The practicability, accuracy, and reliability of the device were further confirmed by using real-time PCR as a gold standard control on the successful identification of 50 processed ground meat samples sourced from local markets. The method and device proposed herein could be a useful tool for on-site identification of food authentication.
基于肉类掺假的食品欺诈行为近来已成为全球主要的经济、非法、宗教及公共卫生问题之一。在本研究中，我们开发了一种微阵列芯片聚合酶链反应（PCR）导向的微流控侧向流动试纸条（LFS）装置，该装置有助于准确且同时鉴定掺有鸡肉、鸭肉和猪肉的牛肉，特别是在加工牛肉制品中。为实现这一目标，设计了四对扩增引物，并用于在微阵列芯片中特异性扩增从混合肉粉中提取的基因组DNA。该装置的突出优势在于其在微观结构中对样品加载、检测和报告的灵活组合与集成，所有DNA扩增产物均可在LFS单元上单独可视化，从而出现测试线（T线、T线、T线或T线）以及用于牛肉制品物种鉴定和定量的对照线（C线）。基于这种新方法，能够成功区分和鉴定混合物中低至0.01%（重量百分比）的掺假物，同时有效避免了常规分子诊断实验室中残留气凝胶污染。通过使用实时PCR作为金标准对照，对从当地市场采集的50个加工碎肉样品进行成功鉴定，进一步证实了该装置的实用性、准确性和可靠性。本文提出的方法和装置可为食品真伪的现场鉴定提供有用工具。
