LMU Munich, Faculty of Medicine, Department of Anatomy II, Pettenkoferstr. 11, 80336, Munich, Germany.
LMU Munich, Faculty of Medicine, Department of Anatomy II, Pettenkoferstr. 11, 80336, Munich, Germany.
Placenta. 2020 Oct;100:66-68. doi: 10.1016/j.placenta.2020.08.003. Epub 2020 Aug 14.
The morphometric parameters of the villous tree are a strong indicator of deviant placentas. Methods have been established to digitally reconstruct small peripheral branches by tracing with 3D Microscopy at subcellular resolution. Micro-CT can help scale up the scanning of villous trees with resolution in the range of a few micrometers. As placental tissue samples are routinely conserved and archived by fixation and paraffin embedding, the villous structures are inaccessible to Micro-CT imaging due to poor contrast between paraffin and paraffinized tissue. We present a novel procedure for contrast enhancement by selectively replacing wax by air in the intervillous space.
绒毛树的形态参数是异常胎盘的一个重要指标。已经建立了通过 3D 显微镜以亚细胞分辨率进行跟踪来对小的外周分支进行数字重建的方法。Micro-CT 可以帮助以几微米范围内的分辨率对绒毛树进行扫描放大。由于胎盘组织样本通常通过固定和石蜡包埋来保存和存档，因此由于石蜡和石蜡化组织之间的对比度差，绒毛结构无法进行 Micro-CT 成像。我们提出了一种通过选择性地用空气替代绒毛间隙中的蜡来增强对比度的新方法。
