Barnard R, Haynes K M, Werther G A, Waters M J
Department of Physiology and Pharmacology, University of Queensland, Australia.
Endocrinology. 1988 Jun;122(6):2562-9. doi: 10.1210/endo-122-6-2562.
To address the question of the mode of action of GH in stimulating longitudinal bone growth, we have used a panel of anti-GH receptor monoclonal antibodies to demonstrate GH receptors in the rabbit tibia and have studied the ontogeny of these receptors. In the neonate, receptors were localized in the hypertrophic zone between the cartilage canals, a region that develops into a secondary ossification center. In support of this finding, receptors were also localized on monolayer cultures of human infant costal chondrocytes. In 20- and 50-day-old rabbits, receptors were localized on reserve and proliferative chondrocytes in the growth plate. In 50- and 130-day-old rabbits receptors were localized on proliferative chondrocytes in the condylar cartilage. In older (180-day-old) rabbits with closed growth plates, GH receptors could not be detected, even in condylar cartilage. These results support the case for revision of the somatomedin hypothesis to accommodate a direct interaction between GH and receptors on epiphyseal chondrocytes.
为了解决生长激素（GH）刺激纵向骨生长的作用方式问题，我们使用了一组抗GH受体单克隆抗体来证明兔胫骨中的GH受体，并研究了这些受体的个体发生情况。在新生儿中，受体定位于软骨管之间的肥大区，该区域会发育成次级骨化中心。支持这一发现的是，受体也定位于人类婴儿肋软骨细胞的单层培养物上。在20日龄和50日龄的兔子中，受体定位于生长板中的储备和增殖软骨细胞上。在50日龄和130日龄的兔子中，受体定位于髁状软骨中的增殖软骨细胞上。在生长板闭合的老年（180日龄）兔子中，即使在髁状软骨中也检测不到GH受体。这些结果支持修订生长介素假说，以适应GH与骨骺软骨细胞上的受体之间的直接相互作用。
