Eden S, Isaksson O G, Madsen K, Friberg U
Endocrinology. 1983 Mar;112(3):1127-9. doi: 10.1210/endo-112-3-1127.
Chondrocytes were isolated from rabbit ear, epiphyseal and rib cartilage, and their ability to bind 125I-labeled human growth hormone (hGH) was investigated. The total binding of hormone to ear chondrocytes increased with time until 4-6 h, whereas non-specific binding did not increase. Total binding was 1-3% of total counts added, and non-specific binding was 10-20% of total binding. Unlabeled hGH, bGH and oPRL competed with the binding of labeled hGH in a dose-dependent manner with half-maximal binding at concentrations of 10-20 ng/ml (hGH) and 100 ng/ml (bGH and oPRL). The two latter hormones displaced almost all specific binding of hGH at higher concentrations. Ear chondrocytes from newborn to 4 week old rabbits all showed specific binding of hGH with no clear age dependence. Rib chondrocytes, on the other hand, showed very little specific binding. Epiphyseal chondrocytes from newborn animals yielded intermediate binding values. The finding of specific hGH binding by cultured chondrocytes supports the concept of a direct effect of GH on chondrocytes.
从兔耳、骨骺和肋软骨中分离出软骨细胞，并研究了它们结合125I标记的人生长激素（hGH）的能力。激素与耳软骨细胞的总结合量随时间增加，直至4 - 6小时，而非特异性结合量没有增加。总结合量为加入总计数的1 - 3%，非特异性结合量为总结合量的10 - 20%。未标记的hGH、bGH和oPRL以剂量依赖方式与标记的hGH结合竞争，在浓度为10 - 20 ng/ml（hGH）和100 ng/ml（bGH和oPRL）时达到半数最大结合。后两种激素在较高浓度时几乎取代了hGH的所有特异性结合。从新生兔到4周龄兔的耳软骨细胞均显示出hGH的特异性结合，且无明显的年龄依赖性。另一方面，肋软骨细胞显示出极少的特异性结合。新生动物的骨骺软骨细胞产生中等结合值。培养的软骨细胞存在特异性hGH结合这一发现支持了GH对软骨细胞有直接作用的概念。
