The First Affiliated Hospital of Lishui University / College of Medicine and Health, Lishui University, Lishui, China.
Pak J Pharm Sci. 2020 Mar;33(2(Supplementary)):835-838.
Triptolide, an ingredient of Tripterygium wilfordii, has been demonstrated to possess many biological activities such as immunomodulatory, antitumor activity in experiment. The purpose of this study was to survey the toxicity of TPL-PEI-CyD on renal cells and its effects on breast carcinoma stem cells. The cytotoxicity of TPL-PEI-CyD and TPL on HK-2 was comparatively assessed by CCK-8. After incubation and culturing with TGF-β1, the MCF-7 cells were assessed by flow cytometry for the proportion of CD CD- cells; then the CD> CD- cells were sorted by immunomagnetic beads as MCF-7 stem cells. To assess the effect of TPL-PEI-CyD on MCF-7 stem cells, Western Blot was used to detect the expression of Oct-4 and ALDHl in MCF-7 stem cells after being dosed with TPL- PEI-CyD. Results showed that, compared with TPL, the toxicity of TPL-PEI-CyD on HK-2 cells was significantly reduced (P<0.05). Breast carcinoma stem cells can be enriched by TGF-β1 and isolated from MCF-7 cells by immunomagnetic sorting. TPL- PEI-CyD can even more significantly suppress the expression of Oct-4 and ALDHA1 in MCF-7 stem cells than TPL (P<0.05). In conclusion, after coupling TPL and PEI-CyD, TPL-PEI-CyD showed characteristics of effective suppression to breast carcinoma stem cell and decrease of cytotoxicity. It presented the unique effect of traditional Chinese medicine as an efficient and low toxic drug carrier complex for breast carcinoma treatment.
雷公藤内酯醇，是从雷公藤中提取的一种成分，已被证明具有许多生物活性，如免疫调节、抗肿瘤活性等。本研究旨在研究 TPL-PEI-CyD 对肾细胞的毒性及其对乳腺癌干细胞的影响。通过 CCK-8 比较 TPL-PEI-CyD 和 TPL 对 HK-2 的细胞毒性。用 TGF-β1 孵育和培养 MCF-7 细胞后，通过流式细胞术评估 CD CD-细胞的比例；然后用免疫磁珠分选 CD> CD-细胞作为 MCF-7 干细胞。为了评估 TPL-PEI-CyD 对 MCF-7 干细胞的影响，用 Western Blot 检测 TPL-PEI-CyD 处理 MCF-7 干细胞后 Oct-4 和 ALDHl 的表达。结果表明，与 TPL 相比，TPL-PEI-CyD 对 HK-2 细胞的毒性明显降低（P<0.05）。乳腺癌干细胞可通过 TGF-β1 富集，并通过免疫磁珠从 MCF-7 细胞中分离。TPL-PEI-CyD 可更显著地抑制 MCF-7 干细胞中 Oct-4 和 ALDHA1 的表达，比 TPL 更显著（P<0.05）。总之，TPL 与 PEI-CyD 偶联后，TPL-PEI-CyD 表现出有效抑制乳腺癌干细胞和降低细胞毒性的特点。它作为一种高效低毒的中药药物载体复合物，为乳腺癌的治疗提供了独特的作用。
