分子印迹聚合物修饰的电位传感器在血清中组胺定量测定中的应用。
Application of Molecularly Imprinted Polymer-modified Potentiometric Sensor for Quantitative Determination of Histamine in Serum.
机构信息
Department of Analytical Chemistry, Kyoto Pharmaceutical University, 5 Misasaginakauchi-cho, Yamashina, Kyoto, 607-8414, Japan.
出版信息
Anal Sci. 2020 Dec 10;36(12):1561-1563. doi: 10.2116/analsci.20N018. Epub 2020 Aug 28.
A molecularly imprinted polymer-modified potentiometric histamine (HIS) sensor was prepared and used for quantitative determination of HIS in bovine serum. The calibration curve using the potential responses measured in 1 × 10 mol L phosphate buffer (pH 7.4) showed good linearity in the HIS concentration range of 3 × 10 to 1 × 10 mol L (r = 0.92), with a detection limit of 1.6 × 10 mol L. In bovine serum samples, the HIS sensor showed good recovery values of 91 - 104%. Therefore, this HIS sensor successfully determined the HIS concentration in bovine serum samples.
一种分子印迹聚合物修饰的电化学组胺（HIS）传感器被制备并用于定量测定牛血清中的 HIS。在 1×10-5 mol/L 磷酸盐缓冲液（pH 7.4）中测量的电势响应的校准曲线在 3×10-5 到 1×10-5 mol/L 的 HIS 浓度范围内表现出良好的线性关系（r=0.92），检测限为 1.6×10-5 mol/L。在牛血清样品中，HIS 传感器显示出 91-104%的良好回收率。因此，该 HIS 传感器成功地测定了牛血清样品中的 HIS 浓度。
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