Culler F L, Kaufmann S, Frigeri L G, Jones K L
Whittier Institute for Diabetes and Endocrinology, University of California, San Diego, La Jolla.
Horm Metab Res. 1988 Feb;20(2):107-9. doi: 10.1055/s-2007-1010764.
The acute metabolic effects of 20,000-dalton human growth hormone (hGH20K) in man have not previously been tested. We compared changes in concentrations of free fatty acids (FFA), glucose, and insulin in nine growth hormone deficient children following injection of 22,000-dalton intact human growth hormone (hGH22K) and the smaller variant, hGH20K. There was a significant decline (37%) in the mean FFA concentration from baseline to 1/2 hour post-injection and from baseline to 1 hour post-injection (36%) in the children given hGH22K, but no such decline was seen after injection of hGH20K. No significant differences in mean insulin or glucose concentrations were noted between the two treatment groups, and glucose and insulin concentrations did not acutely change after injection of either hormone. The results of this study indicate that hGH20K has a diminished activity for suppression of FFA as compared to hGH22K. This suggests that GH residues 32-46, missing in hGH20K, constitute all or part of the region of hGH22K producing this response, or that the different primary structures of the two hormones result in tertiary structural differences and altered biological activity.
20,000道尔顿的人生长激素（hGH20K）对人体的急性代谢作用此前尚未得到测试。我们比较了9名生长激素缺乏儿童在注射22,000道尔顿的完整人生长激素（hGH22K）和较小变体hGH20K后，游离脂肪酸（FFA）、葡萄糖和胰岛素浓度的变化。接受hGH22K治疗的儿童，从基线到注射后半小时平均FFA浓度显著下降（37%），从基线到注射后1小时平均FFA浓度显著下降（36%），但注射hGH20K后未出现这种下降。两个治疗组之间平均胰岛素或葡萄糖浓度没有显著差异，注射任何一种激素后葡萄糖和胰岛素浓度均未急性改变。本研究结果表明，与hGH22K相比，hGH20K抑制FFA的活性降低。这表明hGH20K中缺失的32 - 46位生长激素残基构成了hGH22K产生这种反应的全部或部分区域，或者这两种激素不同的一级结构导致了三级结构差异并改变了生物活性。
