Ma Yihe, Bayguinov Peter O, Jackson Meyer B
Department of Neuroscience, University of Wisconsin - Madison.
Curr Opin Biomed Eng. 2019 Dec;12:51-58. doi: 10.1016/j.cobme.2019.09.007. Epub 2019 Sep 23.
The detection of action potentials and the characterization of their waveform represent basic benchmarks for evaluating optical sensors of voltage. The effectiveness of a voltage sensor in reporting action potentials will determine its usefulness in voltage imaging experiments designed for the study of neural circuitry. The hybrid voltage sensor (hVOS) technique is based on a sensing mechanism with a rapid response to voltage changes. hVOS imaging is thus well suited for optical studies of action potentials. This technique detects action potentials in intact brain slices with an excellent signal-to-noise ratio. These optical action potentials recapitulate voltage recordings with high temporal fidelity. In different genetically-defined types of neurons targeted by cre-lox technology, hVOS recordings of action potentials recapitulate the expected differences in duration. Furthermore, by targeting an hVOS probe to axons, imaging experiments can follow action potential propagation and document dynamic changes in waveform resulting from use-dependent plasticity.
动作电位的检测及其波形特征是评估电压光学传感器的基本标准。电压传感器在报告动作电位方面的有效性将决定其在用于研究神经回路的电压成像实验中的实用性。混合电压传感器（hVOS）技术基于一种对电压变化具有快速响应的传感机制。因此，hVOS成像非常适合用于动作电位的光学研究。该技术能以优异的信噪比在完整脑片中检测动作电位。这些光学动作电位能以高时间保真度重现电压记录。在通过cre-lox技术靶向的不同基因定义类型的神经元中，hVOS对动作电位的记录重现了预期的持续时间差异。此外，通过将hVOS探针靶向轴突，成像实验可以跟踪动作电位的传播，并记录由使用依赖性可塑性导致的波形动态变化。
