Lee Hyeon Jeong, Jiang Ying, Cheng Ji-Xin
College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang 310027.
Department of Electrical and Computer Engineering, Boston University, Boston, MA 02215.
Curr Opin Biomed Eng. 2019 Dec;12:118-125. doi: 10.1016/j.cobme.2019.11.001. Epub 2019 Nov 13.
Offering high temporal resolution, voltage imaging is an important and essential technique in neuroscience. Among different optical imaging approaches, the label-free approach remains attractive due to its unique value coming from free of exogenous chromophores. The intrinsic voltage-indicating signals arising from membrane deformation, membrane spectral change, phase shift, light scattering, and membrane hydration haven been reported. First demonstrated 70 years ago, label-free optical imaging of membrane potential is still at an early stage and the field is challenged by the relatively small signals generated by the intrinsic optical properties. We review major contrast mechanisms used for label-free voltage imaging and discuss several recent exciting advances that could potentially enable membrane potential imaging in mammalian neurons at high speed and high sensitivity.
电压成像具有高时间分辨率，是神经科学中一项重要且必不可少的技术。在不同的光学成像方法中，无标记方法因其无需外源性发色团而具有独特价值，仍然颇具吸引力。已经报道了由膜变形、膜光谱变化、相移、光散射和膜水合作用产生的内在电压指示信号。无标记膜电位光学成像早在70年前就已得到证明，但仍处于早期阶段，该领域面临着由内在光学特性产生的相对较小信号的挑战。我们综述了用于无标记电压成像的主要对比机制，并讨论了几项近期令人兴奋的进展，这些进展有可能实现对哺乳动物神经元膜电位的高速、高灵敏度成像。
