Department of Gamete and Embryo Biology, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, Poland.
Fish Physiology and Genomics, UR1037 (LPGP), INRAE, Rennes, France.
Mol Reprod Dev. 2020 Sep;87(9):934-951. doi: 10.1002/mrd.23414. Epub 2020 Aug 30.
Pikeperch, Sander lucioperca, is a species of high interest to the aquaculture. The expansion of its production can only be achieved by furthering domestication level. However, the mechanisms driving the domestication process in finfishes are poorly understood. Transcriptome profiling of eggs was found to be a useful tool allowing understanding of the domestication process in teleosts. In this study, using next-generation sequencing, the first pikeperch transcriptome has been generated as well as pikeperch-specific microarray comprising 35,343 unique probes. Next, we performed transcriptome profiling of eggs obtained from wild and domesticated populations. We found 710 differentially expressed genes that were linked mostly to nervous system development. These results provide new insights into processes that are directly involved in the domestication of finfishes. It can be suggested that all the identified processes were predetermined by the maternally derived set of genes contained in the unfertilized eggs. This allows us to suggest that fish behavior, along with many other processes, can be predetermined at the cellular level and may have significant implications on the adaptation of cultured fish to the natural environment. This also allows to suggest that fish behavior should be considered as a very important pikeperch aquaculture selection trait.
欧鳊，又称梭鲈，是一种具有高度养殖价值的鱼类。要实现其产量的扩张，只能通过进一步提高其驯化水平来实现。然而，驱动硬骨鱼类驯化过程的机制仍知之甚少。研究发现，对鱼类卵子进行转录组分析是一种有用的工具，可以帮助我们了解硬骨鱼类的驯化过程。在本研究中，我们使用下一代测序技术生成了第一个欧鳊转录组，并构建了包含 35343 个独特探针的欧鳊专用微阵列。接下来，我们对来自野生和驯化种群的卵子进行了转录组分析。我们发现了 710 个差异表达的基因，这些基因主要与神经系统的发育有关。这些结果为直接参与硬骨鱼类驯化的过程提供了新的见解。可以推测，所有鉴定出的过程都是由未受精卵中母体来源的基因决定的。这使我们可以推测，鱼类的行为以及许多其他过程都可以在细胞水平上预先确定，并可能对养殖鱼类适应自然环境产生重大影响。这也使我们可以推测，鱼类的行为应该被视为一个非常重要的欧鳊养殖选择特征。
