Bogar L, Molnar Z, Tekeres M
Department of Anaesthesia and Intensive Therapy, Medical University of Pécs, Hungary.
Immunol Lett. 1988 Mar;17(3):211-5. doi: 10.1016/0165-2478(88)90031-4.
We studied the adaptation of a filtration instrument to an assay for cell migration experiments. The commercially available Nuclepore filter holders were modified so that isolated human polymorphonuclear leucocytes (PMNs) were allowed to penetrate a porous filter membrane and to enter a compartment under the membrane. The total number of PMNs which had passed the pores during an hour of incubation could be counted. In this way we carried out experiments using Shigella sonnei lipopolysaccharide and dilutions of E. coli culture supernatant as chemoattractants for healthy human PMNs. These studies showed that the method can distinguish between random and directed movements of PMNs and it is sensitive to the concentrations of chemoattractant. Furthermore, the data obtained using PMNs from the same subject on different days seem to be comparable.
我们研究了一种过滤仪器用于细胞迁移实验检测的适应性。对市售的核孔滤器支架进行了改良，以便使分离出的人多形核白细胞（PMN）能够穿透多孔滤膜并进入膜下隔室。可以对孵育一小时内通过孔的PMN总数进行计数。通过这种方式，我们使用宋内志贺氏菌脂多糖和大肠杆菌培养上清液稀释液作为健康人PMN的趋化因子进行了实验。这些研究表明，该方法能够区分PMN的随机运动和定向运动，并且对趋化因子的浓度敏感。此外，在不同日期使用同一受试者的PMN获得的数据似乎具有可比性。
