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艰难梭菌分泌抗原在大肠杆菌中的克隆与表达。

Cloning and expression of secreted antigens of Clostridium difficile in Escherichia coli.

作者信息

Dailey D C, Schloemer R H

机构信息

Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis 46223.

出版信息

Infect Immun. 1988 Jun;56(6):1655-7. doi: 10.1128/iai.56.6.1655-1657.1988.

DOI:10.1128/iai.56.6.1655-1657.1988
PMID:3286505
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC259452/
Abstract

The feasibility of the cloning and expression of Clostridium difficile antigens in Escherichia coli was investigated. The expression of a limited number of cloned clostridial antigens under the control of clostridial promoter elements in E. coli was observed.

摘要

研究了艰难梭菌抗原在大肠杆菌中克隆和表达的可行性。观察到在大肠杆菌中，有限数量的克隆梭菌抗原在梭菌启动子元件控制下的表达情况。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a74/259452/2e45b73cc655/iai00078-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a74/259452/34b2831f77e4/iai00078-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a74/259452/2e45b73cc655/iai00078-0258-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a74/259452/34b2831f77e4/iai00078-0257-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2a74/259452/2e45b73cc655/iai00078-0258-a.jpg

相似文献

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本文引用的文献

1
Clostridium difficile in gnotobiotic mice.悉生小鼠中的艰难梭菌。
Infect Immun. 1980 Apr;28(1):277-82. doi: 10.1128/iai.28.1.277-282.1980.
2
Presence of Clostridium difficile toxin in guinea pigs with penicillin-associated colitis.
Med Microbiol Immunol. 1981;169(3):187-96. doi: 10.1007/BF02123592.
3
Myoelectric effects of Clostridium difficile: motility-altering factors distinct from its cytotoxin and enterotoxin in rabbits.艰难梭菌的肌电效应：家兔体内不同于其细胞毒素和肠毒素的运动改变因子。
Gastroenterology. 1982 Oct;83(4):836-43.
4
Purification and characterization of toxins A and B of Clostridium difficile.艰难梭菌毒素A和毒素B的纯化与特性分析
Infect Immun. 1982 Mar;35(3):1032-40. doi: 10.1128/iai.35.3.1032-1040.1982.
5
Pseudomembranous colitis in Clostridium difficile-monoassociated rats.艰难梭菌单关联大鼠的假膜性结肠炎
Infect Immun. 1983 Mar;39(3):1368-76. doi: 10.1128/iai.39.3.1368-1376.1983.
6
Inciting and etiologic agents of colitis.结肠炎的诱发因素和病因
Rev Infect Dis. 1984 Mar-Apr;6 Suppl 1:S214-21. doi: 10.1093/clinids/6.supplement_1.s214.
7
Biochemical characterization and biologic actions of two toxins (D-1 and D-2) from Clostridium difficile.艰难梭菌两种毒素（D-1和D-2）的生化特性及生物学作用
Rev Infect Dis. 1984 Mar-Apr;6 Suppl 1:S11-20. doi: 10.1093/clinids/6.supplement_1.s11.
8
Antimicrobial agent-associated colitis and diarrhea: historical background and clinical aspects.抗菌药物相关性结肠炎和腹泻：历史背景与临床方面
Rev Infect Dis. 1984 Mar-Apr;6 Suppl 1:S208-13. doi: 10.1093/clinids/6.supplement_1.s208.
9
Studies on transformation of Escherichia coli with plasmids.大肠杆菌质粒转化的研究。
J Mol Biol. 1983 Jun 5;166(4):557-80. doi: 10.1016/s0022-2836(83)80284-8.
10
Cloning and expression of Legionella pneumophila antigens in Escherichia coli.嗜肺军团菌抗原在大肠杆菌中的克隆与表达。
Infect Immun. 1984 May;44(2):222-7. doi: 10.1128/iai.44.2.222-227.1984.