Lyerly D M, Ball D W, Toth J, Wilkins T D
Department of Anaerobic Microbiology, Virginia Polytechnic Institute and State University, Blacksburg 24061.
J Clin Microbiol. 1988 Mar;26(3):397-400. doi: 10.1128/jcm.26.3.397-400.1988.
Clostridium sporogenes, Peptostreptococcus anaerobius, and Bacteroides asaccharolyticus have been reported to react in the Culturette Brand Rapid Latex Test (Marion Scientific, Div. Marion Laboratories, Inc., Kansas City, Mo.) for Clostridium difficile. From the results of this study we showed that C. sporogenes and P. anaerobius produce a protein which is very similar biochemically and immunologically to the protein of C. difficile that is detected by the test. Thus, the positive latex reactions observed with C. sporogenes and P. anaerobius are due to a cross-reactive protein. We did not detect this cross-reactive protein in filtrates from B. asaccharolyticus, indicating that this bacterium reacts with the latex reagent by some other mechanism. We cloned the C. difficile gene that codes for the cross-reactive protein and showed that the protein produced by the recombinant organism is nontoxic and distinct from toxin A, thus confirming our earlier findings.
据报道，生孢梭菌、厌氧消化链球菌和不解糖拟杆菌在用于艰难梭菌检测的Culturette品牌快速乳胶试验（马里恩科学公司，马里恩实验室分部，密苏里州堪萨斯城）中会产生反应。从本研究结果来看，我们发现生孢梭菌和厌氧消化链球菌产生一种蛋白质，该蛋白质在生化和免疫方面与该试验检测到的艰难梭菌蛋白质非常相似。因此，生孢梭菌和厌氧消化链球菌出现的阳性乳胶反应是由于一种交叉反应蛋白。我们在不解糖拟杆菌的滤液中未检测到这种交叉反应蛋白，这表明该细菌通过其他某种机制与乳胶试剂发生反应。我们克隆了编码这种交叉反应蛋白的艰难梭菌基因，并表明重组生物体产生的蛋白质无毒且与毒素A不同，从而证实了我们早期的发现。
