Department of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Gujarat, Vadodara, India.
Department of Reconstructive Surgery, Solace Hospital, Vadodara, India.
Immunol Invest. 2022 Jan;51(1):120-137. doi: 10.1080/08820139.2020.1813756. Epub 2020 Aug 30.
Interleukin-6 (IL6) is involved in pathogenesis of several autoimmune disorders including vitiligo. Hence, we aimed to investigate the association of IL6 -174 G/C and -572 G/C polymorphisms and its transcript levels with vitiligo; to evaluate the effect of IL-6 on normal human melanocyte (NHM) viability and expression of IL6R, MITF and TYR. IL6 -174 G/C and -572 G/C polymorphisms were genotyped by ARMS-PCR and PCR-RFLP respectively in 343 controls and 322 vitiligo patients. IL6 transcript levels were estimated from PBMCs (96 controls and 77 patients) and skin samples (15 controls and 15 patients) by qPCR. NHM viability was assessed by MTT; IL6R, MITF and TYR transcript and protein levels were monitored by qPCR and ICC respectively. Genetic analyses revealed no association of IL6 -174 G/C polymorphism (p> .05) with vitiligo. Analysis of IL6 -572 G/C revealed reduced risk of vitiligo in individuals with GC/CC genotypes compared to GG genotype (p = .010). IL6 expression was significantly increased (p = .0197) in PBMCs of patients. Further, IL6 expression was significantly higher in non-lesional skin compared to controls (p = .009). In-vitro NHM viability was decreased upon IL-6 exposure (10-50 ng/ml; p< .05), with significantly increased IL6R transcript (p = .042) and protein levels (p = .003) however, MITF transcript (p = .0003) and protein levels (p = .016), and TYR transcript levels (p = .001) were significantly decreased. The results suggest that IL6 -572 G/C polymorphism might be associated with vitiligo susceptibility in Gujarat population. Moreover, increased IL6 expression in vitiligo patients and its effect on NHM suggest a potential role in melanocyte biology.
The results suggest that 72 G/C polymorphism might be associated with vitiligo susceptibility in Gujarat population. Moreover, increased expression in vitiligo patients and its effect on NHM suggest a potential role in melanocyte biology.
白细胞介素-6（IL6）参与多种自身免疫性疾病的发病机制，包括白癜风。因此，我们旨在研究 IL6-174G/C 和-572G/C 多态性及其转录水平与白癜风的关系；评估 IL-6 对正常人黑素细胞（NHM）活力和 IL6R、MITF 和 TYR 表达的影响。通过 ARMS-PCR 和 PCR-RFLP 分别对 343 名对照者和 322 名白癜风患者的 IL6-174G/C 和-572G/C 多态性进行基因分型。通过 qPCR 从 PBMC（96 名对照者和 77 名患者）和皮肤样本（15 名对照者和 15 名患者）中估算 IL6 转录水平。通过 MTT 评估 NHM 活力；通过 qPCR 和 ICC 分别监测 IL6R、MITF 和 TYR 转录和蛋白水平。遗传分析显示 IL6-174G/C 多态性（p>.05）与白癜风无关。IL6-572G/C 分析显示，与 GG 基因型相比，GC/CC 基因型的白癜风发病风险降低（p=0.010）。患者 PBMC 中 IL6 的表达显著增加（p=0.0197）。此外，非病变皮肤中的 IL6 表达明显高于对照组（p=0.009）。体外 NHM 活力在 IL-6 暴露（10-50ng/ml；p<.05）后下降，IL6R 转录（p=0.042）和蛋白水平（p=0.003）显著增加，然而，MITF 转录（p=0.0003）和蛋白水平（p=0.016）以及 TYR 转录水平（p=0.001）显著降低。结果表明，IL6-572G/C 多态性可能与古吉拉特邦人群的白癜风易感性有关。此外，白癜风患者中 IL6 的表达增加及其对 NHM 的影响表明其在黑素细胞生物学中可能具有潜在作用。
结果表明，72G/C 多态性可能与古吉拉特邦人群的白癜风易感性有关。此外，白癜风患者中 IL6 的表达增加及其对 NHM 的影响表明其在黑素细胞生物学中可能具有潜在作用。
