Department of Neurosurgery & Brain and Nerve Research Laboratory, The First Affiliated Hospital of Soochow University, 188 Shizi Street, Suzhou, 215006, Jiangsu Province, China.
Neuromolecular Med. 2021 Jun;23(2):267-284. doi: 10.1007/s12017-020-08611-5. Epub 2020 Aug 31.
Intracerebral hemorrhage (ICH) is a devastating disease with high rates of mortality and morbidity. Galactose lectin-9 (Gal-9) belongs to the family of β-galactoside-binding lectins, which has been shown to play a vital role in immune tolerance and inflammation. However, the function of Gal-9 in ICH has not been fully studied in details. Several experiments were carried out to explore the role of Gal-9 in the late period of ICH. Primarily, ICH models were established in male adult Sprague Dawley (SD) rats. Next, the relative protein levels of Gal-9 at different time points after ICH were examined and the result showed that the level of Gal-9 increased and peaked at the 7th day after ICH. Then we found that when the content of Gal-9 increased, both the number of M2-type microglia and the corresponding anti-inflammatory factors also increased. Through co-immunoprecipitation (CO-IP) analysis, it was found that Gal-9 combines with Toll-like receptor-4 (TLR-4) during the period of the recovery after ICH. TUNEL staining and Fluoro-Jade B staining (FJB) proved that the amount of cell death decreased with the increase of Gal-9 content. Additionally, several behavioral experiments also demonstrated that when the level of Gal-9 increased, the motor, sensory, learning, and memory abilities of the rats recovered better compared to the ICH group. In short, this study illustrated that Gal-9 takes a crucial role after ICH. Enhancing Gal-9 could alleviate brain injury and promote the recovery of ICH-induced injury, so that Gal-9 may exploit a new pathway for clinical treatment of ICH.
脑出血（ICH）是一种死亡率和发病率都很高的破坏性疾病。半乳糖凝集素-9（Gal-9）属于β-半乳糖苷结合凝集素家族，它在免疫耐受和炎症中起着至关重要的作用。然而，Gal-9 在 ICH 中的功能尚未被充分研究。进行了几项实验来探索 Gal-9 在 ICH 后期的作用。首先，在雄性成年 Sprague Dawley（SD）大鼠中建立 ICH 模型。接下来，检测了 ICH 后不同时间点 Gal-9 的相对蛋白水平，结果表明 Gal-9 水平在 ICH 后第 7 天增加并达到峰值。然后我们发现，当 Gal-9 含量增加时，M2 型小胶质细胞的数量和相应的抗炎因子也增加。通过共免疫沉淀（CO-IP）分析，发现 Gal-9 在 ICH 后恢复期间与 Toll 样受体-4（TLR-4）结合。TUNEL 染色和 Fluoro-Jade B 染色（FJB）证明细胞死亡的数量随着 Gal-9 含量的增加而减少。此外，几项行为学实验也表明，当 Gal-9 水平增加时，与 ICH 组相比，大鼠的运动、感觉、学习和记忆能力恢复得更好。总之，这项研究表明 Gal-9 在 ICH 后起着至关重要的作用。增强 Gal-9 可以减轻脑损伤并促进 ICH 诱导损伤的恢复，因此 Gal-9 可能为 ICH 的临床治疗开辟新途径。
