Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Brain Function and Disease, School of Life Sciences, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, Anhui, 230026, China.
Department of Biology and Hong Kong Branch of the Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou), Hong Kong Baptist University, Hong Kong, China.
Proteomics. 2020 Oct;20(19-20):e2000167. doi: 10.1002/pmic.202000167. Epub 2020 Oct 4.
Sperm proteins play vital roles in fertilization, but little is known about their identities in free-spawning marine invertebrates. Here, 286 sperm proteins are reported from the Hong Kong oyster Crassostrea hongkongensis using label-free and semi-quantitative proteomics. Proteins extracted from three sperm samples are separated by SDS-PAGE, analyzed by LC-MS/MS, and identified using Mascot. Functional classification of the sperm proteome reveals energy metabolism (33%), signaling and binding (23%), and protein synthesis and degradation (12%) as the top functional categories. Comparison of orthologous sperm proteins between C. hongkongensis, Crassostrea gigas, Mytilus edulis, and M. galloprovincialis suggests that energy metabolism (48%) is the most conserved functional group. Sequence alignment of the C. hongkongensis bindin, an acrosomal protein that binds the sperm and the egg, with those of three other Crassostrea species, reveals several conserved motifs. The study has enriched the data of invertebrate sperm proteins and may contribute to studies of mechanisms of fertilization in free-spawning invertebrates. The proteomic data are available in ProteomeXchange with the identifier PXD018255.
精子蛋白在受精过程中起着至关重要的作用，但人们对自由产卵的海洋无脊椎动物中的精子蛋白的身份知之甚少。本研究采用无标记和半定量蛋白质组学方法，从香港牡蛎（Crassostrea hongkongensis）中鉴定到 286 种精子蛋白。从 3 个精子样本中提取的蛋白质经 SDS-PAGE 分离，用 LC-MS/MS 进行分析，并用 Mascot 进行鉴定。精子蛋白组的功能分类揭示了能量代谢（33%）、信号和结合（23%）以及蛋白质合成和降解（12%）为顶级功能类别。对香港牡蛎、中国蛤蜊（Crassostrea gigas）、贻贝（Mytilus edulis）和马氏珠母贝（M. galloprovincialis）的同源精子蛋白进行比较表明，能量代谢（48%）是最保守的功能群。香港牡蛎顶体结合蛋白（一种与精子和卵子结合的顶体蛋白）与其他三种中国蛤蜊物种的序列比对显示出几个保守的基序。本研究丰富了无脊椎动物精子蛋白的数据，可能有助于研究自由产卵无脊椎动物的受精机制。蛋白质组学数据可在 ProteomeXchange 中以标识符 PXD018255 获得。
