Cohen-Krais Jayme, Martins Carlo, Bartram Jamie, Crighton Zoe, de Coriolis Jean-Charles, Godden Alice, Marcu Daniel, Robak Weronika, Saalbach Gerhard, Immler Simone
School of Biological Sciences, University of East Anglia, Norwich Research Park, Norwich, Norfolk, UK.
Biochemistry & Metabolism, John Innes Centre, Norwich Research Park, Norwich, Norfolk, UK.
Proteomics. 2025 Feb;25(4):e202400310. doi: 10.1002/pmic.202400310. Epub 2024 Dec 31.
One of the key processes that forms the basis of fertilisation is the tight interaction between sperm and egg. Both sperm and egg proteomes are known to evolve and diverge rapidly even between closely related species. Understanding the sperm proteome therefore provides key insights into the proteins that underpin the mechanisms involved during fertilisation and the fusion between sperm and egg, and how they can differ across individuals of the same species. Despite being a commonly used model organism for reproductive research, little is currently understood about the zebrafish Danio rerio sperm proteome. We performed nanoLC-MS/MS proteomics analysis after off-line sample fractionation with six pooled samples containing sperm from ten males each. We confidently identified 5410 proteins, from which a total of 3900 GeneIDs were generated leading to 1720 Gene Ontology terms.
构成受精基础的关键过程之一是精子与卵子之间的紧密相互作用。已知精子和卵子的蛋白质组即使在亲缘关系很近的物种之间也会迅速进化和分化。因此，了解精子蛋白质组有助于深入了解那些支撑受精过程以及精子与卵子融合机制的蛋白质，以及它们在同一物种的不同个体之间可能存在的差异。尽管斑马鱼是生殖研究中常用的模式生物，但目前对其精子蛋白质组的了解甚少。我们在离线样品分级分离后，对六个合并样本进行了纳升液相色谱-串联质谱蛋白质组学分析，每个合并样本包含来自十只雄性斑马鱼的精子。我们可靠地鉴定出了5410种蛋白质，从中总共生成了3900个基因ID，对应1720个基因本体术语。
