Noel A, Callé A, Emonard H, Nusgens B, Foidart J M, Lapiere C M
Laboratory of Experimental Dermatology and Pathophysiology of Pregnancy, University of Liège, Belgium.
In Vitro Cell Dev Biol. 1988 May;24(5):373-80. doi: 10.1007/BF02628488.
During morphogenesis, tumor progression and metastasis, cell adhesion, dissociation, and migration result from a complex balance between cell-to-cell and cell-to-matrix interactions. Two different organization patterns of MCF-7 cells were induced by different extracellular matrix proteins. When plated on plastic or polymeric type I collagen gel used as a model of interstitial matrix, MCF-7 cells spread and grew in monolayer. When cultured on a solid gel of basement membrane (BM) proteins (85% laminin) used as a model of BM, cells formed clusters attached to the matrix. Matrix proteins regulated these two types of cell organization by preferentially promoting cell-to-cell or cell-support interactions. On plastic in the presence of soluble laminin or on laminin-coated dishes, cells also formed clusters. Addition of soluble fibronectin induced spreading of the cells, suggesting that laminin and fibronectin have competitive antagonistic effects on MCF-7 cell morphology. Antilaminin antibodies inhibited cluster formation and attachment, emphasizing the important role of this glycoprotein not only in promoting cluster attachment but also in cell-to-cell contact formation. Such effects of extracellular matrix proteins could play significant roles in tumor progression and metastasis.
在形态发生、肿瘤进展和转移过程中，细胞粘附、解离和迁移源于细胞间以及细胞与基质相互作用之间的复杂平衡。不同的细胞外基质蛋白诱导了MCF-7细胞的两种不同组织模式。当接种在用作间质基质模型的塑料或聚合I型胶原凝胶上时，MCF-7细胞铺展并单层生长。当在用作基底膜（BM）模型的基底膜（BM）蛋白（85%层粘连蛋白）固体凝胶上培养时，细胞形成附着于基质的簇。基质蛋白通过优先促进细胞间或细胞-支持物相互作用来调节这两种细胞组织类型。在存在可溶性层粘连蛋白的塑料上或在层粘连蛋白包被的培养皿上，细胞也形成簇。添加可溶性纤连蛋白诱导细胞铺展，表明层粘连蛋白和纤连蛋白对MCF-7细胞形态具有竞争性拮抗作用。抗层粘连蛋白抗体抑制簇的形成和附着，强调了这种糖蛋白不仅在促进簇附着方面而且在细胞间接触形成方面的重要作用。细胞外基质蛋白的这种作用可能在肿瘤进展和转移中发挥重要作用。
