Sukegawa I, Hizuka N, Takano K, Asakawa K, Horikawa R, Hashida S, Ishikawa E, Mohri Z, Murakami Y, Shizume K
Department of Medicine, Tokyo Women's Medical College, Japan.
J Clin Endocrinol Metab. 1988 Jun;66(6):1119-23. doi: 10.1210/jcem-66-6-1119.
Daily (24-h) urinary GH excretion was measured using a highly sensitive sandwich enzyme immunoassay in 10 normal adults, 6 patients with hypopituitarism, 25 normal but short children who had normal plasma GH responses (peak plasma GH level, greater than 10 micrograms/L) to provocative tests, and 8 patients with acromegaly. The mean urinary GH values in the normal adults, patients with acromegaly, and patients with hypopituitarism were 13.8 +/- 4.0 (+/- SE) and 431.1 +/- 149.1 ng/g creatinine (Cr) (1.56 +/- 0.45 and 48.77 +/- 16.87 ng/mmol Cr) and undetectable, respectively; these mean values were significantly different from each other. In the normal but short children the urinary values ranged from undetectable to 55.8 ng/g Cr (6.31 ng/mmol Cr). All of the normal but short children and 4 patients with hypopituitarism participated in a 24-h endogenous GH secretion study. The urinary GH values correlated significantly with the mean 24-h plasma GH concentrations as an index of endogenous GH secretion (r = 0.81; P less than 0.001) and plasma somatomedin-C levels (r = 0.67; P less than 0.001), respectively. In 6 patients with acromegaly whose plasma GH levels were constant throughout a 4-h period, the urinary GH values also significantly correlated with the mean plasma GH levels (r = 0.95; P less than 0.01). These data indicate that urinary GH measurements reflect endogenous GH secretion and that measurement of urinary GH excretion is a useful, simple, and practical method for evaluating endogenous GH secretion.
采用高灵敏度夹心酶免疫分析法，对10名正常成年人、6名垂体功能减退患者、25名血浆生长激素（GH）对激发试验反应正常（血浆GH峰值水平大于10微克/升）的正常但身材矮小儿童以及8名肢端肥大症患者，测定其每日（24小时）尿GH排泄量。正常成年人、肢端肥大症患者和垂体功能减退患者的尿GH平均水平分别为13.8±4.0（±标准误）和431.1±149.1纳克/克肌酐（Cr）（1.56±0.45和48.77±16.87纳克/毫摩尔Cr），垂体功能减退患者尿GH水平未检测到；这些平均值彼此差异显著。正常但身材矮小的儿童尿GH值范围为未检测到至55.8纳克/克Cr（6.31纳克/毫摩尔Cr）。所有正常但身材矮小的儿童和4名垂体功能减退患者参与了一项24小时内源性GH分泌研究。尿GH值分别与作为内源性GH分泌指标的24小时血浆GH平均浓度（r = 0.81；P<0.001）和血浆生长调节素C水平（r = 0.67；P<0.001）显著相关。在6名血浆GH水平在4小时内保持恒定的肢端肥大症患者中，尿GH值也与血浆GH平均水平显著相关（r = 0.95；P<0.01）。这些数据表明，尿GH测量反映内源性GH分泌，并且测定尿GH排泄量是评估内源性GH分泌的一种有用、简单且实用的方法。
