Senyk G F, Goodall C, Kozlowski S M, Bandler D K
Cornell University, Ithaca, NY 14853.
J Dairy Sci. 1988 Mar;71(3):613-9. doi: 10.3168/jds.S0022-0302(88)79598-3.
Raw milk samples were collected from 10 producer bulk tanks. Samples were then subdivided so that milks were subsequently stored at 1.7, 4.4, 7.2, and 10.0 degrees C for 24 and 48 h. After storage, samples were analyzed by seven plating methods: standard plate count, psychrotrophic bacterial count, rapid psychrotrophic count, preliminary incubation count, mesophilic plate count, laboratory pasteurized count, and coliform count by violet red bile agar technique. Impedance protocols on a Bactometer Model 123 for total count, psychrotrophic count, mesophilic count, and coliform count were also used to evaluate the bacteriological quality of the milks. Bacterial counts and impedance detection times were analyzed using nonparametric statistics. Impedance protocols for total count and psychrotrophic count were the best indicators of bacteriological quality. Preliminary incubation count was the best of the plating methods. The laboratory pasteurized count performed poorly. Impedance measurements provided information in the shortest time.
从10个生产商的大容量奶罐中采集了生乳样本。然后将样本进行细分,以便后续将牛奶分别储存在1.7、4.4、7.2和10.0摄氏度下24小时和48小时。储存后,通过七种平板计数法对样本进行分析:标准平板计数、嗜冷菌计数、快速嗜冷菌计数、预培养计数、嗜温平板计数、实验室巴氏杀菌计数以及采用紫红胆盐琼脂技术的大肠菌群计数。还使用Bactometer Model 123的阻抗方案对总数、嗜冷菌数、嗜温菌数和大肠菌群数进行评估,以评价牛奶的细菌学质量。使用非参数统计分析细菌计数和阻抗检测时间。总数和嗜冷菌计数的阻抗方案是细菌学质量的最佳指标。预培养计数是平板计数法中最好的。实验室巴氏杀菌计数表现不佳。阻抗测量能在最短时间内提供信息。