Department of Genetic Resources, National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea.
Marine-Integrated Bionics Research Center, Pukyong National University, Busan 48513, Korea.
Nutrients. 2020 Aug 27;12(9):2620. doi: 10.3390/nu12092620.
Acne vulgaris is a chronic inflammatory condition of skin sebaceous follicles. To explore its effects on acne vulgaris, we investigated the antibacterial and anti-inflammatory activities of Yendo (a brown alga) ethanolic extract (SMYEE) on ()-stimulated inflammatory responses, both in vivo and in vitro. To induce inflammation in vivo, was intradermally injected into the dorsal skin of mice, to which SMYEE was applied. The antimicrobial activity of SMYEE was evaluated by the determination of minimum inhibitory concentrations (MICs). To explore in vitro anti-inflammatory effects, HaCaT cells were stimulated with after treatment with SMYEE. The levels of IL-8 and the underlying molecular effects in -stimulated HaCaT cells were assessed by enzyme-linked immunosorbent assay, Western blotting, and an electrophoretic mobility shift assay. Mouse skin lesions improved after treatment with SMYEE (50 μg/mouse). Neutrophil infiltration was significantly reduced in SMYEE-treated compared to SMYEE-untreated skin lesions. SMYEE reversed the -induced increase in IL-8 levels in HaCaT cells and suppressed dHL-60 cell migration. SMYEE also inhibited -induced phosphorylation of the extracellular signal-regulated kinase and inhibited activator protein-1 signaling. SMYEE may be a useful treatment for -induced acne vulgaris.
寻常痤疮是一种皮肤皮脂腺滤泡的慢性炎症性疾病。为了探讨其对寻常痤疮的影响，我们研究了 Yendo（一种褐藻）乙醇提取物（SMYEE）对（）刺激的炎症反应的体外和体内的抗菌和抗炎活性。为了在体内诱导炎症，将 SMYEE 应用于小鼠背部皮肤的真皮内注射。通过测定最小抑菌浓度（MIC）来评估 SMYEE 的抗菌活性。为了探讨体外抗炎作用，用 SMYEE 处理后用刺激 HaCaT 细胞。通过酶联免疫吸附试验、Western blot 和电泳迁移率变动分析评估 SMYEE 处理后 - 刺激的 HaCaT 细胞中白细胞介素-8（IL-8）水平及其潜在的分子作用。用 SMYEE（50 μg/只）处理后，小鼠皮肤病变得到改善。与未用 SMYEE 处理的皮肤病变相比，中性粒细胞浸润在 SMYEE 处理的皮肤病变中明显减少。SMYEE 逆转了 - 诱导的 HaCaT 细胞中 IL-8 水平的增加，并抑制了 dHL-60 细胞的迁移。SMYEE 还抑制了 - 诱导的细胞外信号调节激酶磷酸化，并抑制了激活蛋白-1 信号转导。SMYEE 可能是一种有用的治疗方法，用于治疗由 - 引起的寻常痤疮。
